Application Data
(Fig.Fluo-4 Calcium Assay Kit)
Micro Dehydroascorbic Acid (DHA) Assay Kit | DHA assay kit
Micro Dehydroascorbic Acid (DHA) Assay Kit
Synonyms
Micro Dehydroascorbic Acid (DHA); N/A; Micro Dehydroascorbic Acid (DHA) Assay Kit; DHA; DHA assay kit
Samples
Cells
Usage Notes
If a medium containing serum is used, the esterase in the serum will hydrolyze Fluo-4 AM, thus reducing the effectiveness of Fluo-4 AM in entering cells. In addition, the medium containing phenol red will make the background value slightly higher, and the residual medium should be removed as far as possible before adding the Stain Solution.
Ionomycin is an ionic vector with affinity for calcium. The increase in green fluorescence intensity can be observed within a few seconds after Ionomycin treatment. It is recommended to use laser confocal microscope to continuously take photos to detect the change of intracellular calcium concentration.
Ionomycin is an ionic vector with affinity for calcium. The increase in green fluorescence intensity can be observed within a few seconds after Ionomycin treatment. It is recommended to use laser confocal microscope to continuously take photos to detect the change of intracellular calcium concentration.
Preparation and Storage
Stored at -20 degree C for 12 months, protected from light
Application Data
(Fig.Fluo-4 Calcium Assay Kit)
Application Data
(Fig.Fluo-4 Calcium Assay Kit)
Related Product Information for DHA assay kit
Principle of the Assay: Fluo-4 Calcium Assay Kit is a kit for detecting intracellular calcium concentration based on Fluo-4 AM calcium fluorescent probe. This kit can be used for fluorescence imaging detection using a fluorescence microscopy, or quantitative fluorescence detection using florescence microplate reader or flow cytometry. Using fluorescence microscopy or fluorescence microplate reader can also detect the dynamic changes of the intracellular calcium concentration. Fluo-4 is a calcium fluorescence probe that replaces Cl in the Fluo-3 structure with F. Due to the replacement of Cl with F with stronger electronic attraction, its maximum excitation wavelength will deviate by about 10 nm towards the shorter wavelength direction. This wavelength is closer to the wavelength of an argon laser, so when excited by an argon laser, the fluorescence intensity of Fluo-4 is stronger than that of Fluo-3. Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell. When Fluo-4 exists as a free ligand, it is almost non fluorescent, but when combined with intracellular calcium, it can produce strong fluorescence. Laser confocal microscopy can be used to detect changes in intracellular calcium concentration.
Product Categories/Family for DHA assay kit
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Product Notes
The DHA (Catalog #AAA332617) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Micro Dehydroascorbic Acid (DHA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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