MTT-Cell Based Proliferation/Toxicity Assay Kit | MTT assay kit
MTT-Cell Based Proliferation/Toxicity Assay Kit
Synonyms
MTT-Cell Based Proliferation/Toxicity; N/A; MTT-Cell Based Proliferation/Toxicity Assay Kit; MTT assay kit
Samples
MTT is carcinogenic. Avoid direct contact. Use gloves and eye protection
Related Product Information for MTT assay kit
Background: Measurement of cell viability and proliferation forms the basis for numerous in vitro assays of a cell population's response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable way to examine cell proliferation. The key substrate of the kit is:3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide or MTT. MTT is a yellowish solution when dissolved in balanced salt solutions without phenol red and is taken up by cells due to its net positive charge. The tetrazolium ring of MTT (yellow) is reduced to purple formazan crystals by intracellular NAD(P)H-oxidoreductases. The formazan crystals are insoluble in aqueous solution, but become solubilized in the provided solubilization Buffer. After solubilization, the resulting purple solution is spectrophotometrically measured. The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction in cell viability. The number of assay steps has been minimized as much as possible to expedite sample processing. The MTT solution yields low background absorbance values in the absence of cells. For each cell type the linear relationship between cell number and signal produced is established, thus allowing an accurate quantification of changes in the rate of cell proliferation.
Similar Products
Product Notes
The MTT (Catalog #AAA249418) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MTT-Cell Based Proliferation/Toxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.