NAD+ kinase (NADK) Assay Kit | NADK assay kit
NAD+ kinase (NADK) Activity Colorimetric Assay Kit
Synonyms
NAD+ kinase (NADK); N/A; NAD+ kinase (NADK) Activity Colorimetric Assay Kit; NADK assay kit
Samples
Serum, Plasma, Animal and Plant Tissues, Cells, Bacteria
Features & Benefits
Determination of NADK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
Detailed sample preparation and result calculation methods are provided.
Simple operation steps.
Detailed sample preparation and result calculation methods are provided.
Simple operation steps.
Usage Notes
96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
Do not mix or substitute reagents or materials from other kit lots or vendors.
The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
Substrate Mix and Enzyme Mix must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25 degree C (general species) or 37 degree C (mammal).
Do not mix or substitute reagents or materials from other kit lots or vendors.
The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
Substrate Mix and Enzyme Mix must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25 degree C (general species) or 37 degree C (mammal).
Preparation and Storage
Storage at -20 degree C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Related Product Information for NADK assay kit
Principle of the Assay: NAD+ Kinase (NADK, EC 2.7.1.23) is widely found in animals, plants, microorganisms and cells. It is the only enzyme that can catalyze the Phosphorylation of NAD+ to NADP+ in organisms. It can catalyze the Phosphorylation reaction of NAD(H) with ATP or inorganic Polyphosphate [poly(P)] as a Phosphoryl donor to generate NADP(H). Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H). CheKineTM NAD+ Kinase (NADK) Activity Colorimetric Assay Kit provides a simple method for detecting NADK activity in a variety of biological samples such as Serum, Plasma, Animal and Plant Tissues, Cells, Bacteria. In the assay, NADK catalyzes the Phosphorylation of NAD+ to produce NADP+; NADP+ can be reduced to NADPH by Glucose-6-Phosphate Dehydrogenase. NADPH has a characteristic absorption peak at 340 nm. The rate of NADPH increase at 340 nm can reflect NADK activity.
Product Categories/Family for NADK assay kit
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Product Notes
The NADK (Catalog #AAA332478) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NAD+ kinase (NADK), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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