Standard Curve (Sample)
NAD/NADH assay kit
NAD/NADH Assay Kit
Synonyms
NAD/NADH; N/A; NAD/NADH Assay Kit; NAD/NADH assay kit
Samples
Animal Tissues, Cells, Bacteria
Detection Range
0.78 uM-50 uM
Sensitivity
0.78 uM
Preparation and Storage
Storage at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NAD/NADH assay kit
Principle of the Assay: Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all cells, including two forms, NAD+(oxidized) and NADH (reduced). NAD+ is not only a coenzyme that transfers electrons during REDOX reaction, but also a substrate for many enzymes to participate in intracellular reactions. NAD+ plays an important role in cells and in vivo. Its synthesis, degradation and its products are participated in apoptosis, metabolism regulation and gene expression regulation, and the reduction of NAD+ is one of the main factors of cell death. The importance of NAD+ in regulating the REDOX state of cells and its function in regulating signaling pathways and transcription make NAD+, its synthesis and consumption enzymes to be potential drug targets for a variety of diseases. CheKine™ CoenzymeI NAD(H) Colorimetric Assay Kit is a WST-8 based chromogenic reaction that uses colorimetry to measure the amount, ratio, and total amount of NAD+(oxidized coenzyme I) and NADH(reduced Coenzyme I) in cells, tissues, or other samples. The NAD+/NADH assay kit is based on an enzyme cycle reaction (which does not recognize NADP+/NADPH) in which NAD+ is reduced to NADH and NADH reduces WST-8 to orange formazan with a maximum absorption peak detected at around 450 nm. The formation of Formazan in the reaction system is proportional to the total amount of NAD+ or NADH in the sample.
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Product Notes
The NAD/NADH (Catalog #AAA330131) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NAD/NADH, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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