NADP+/NADPH assay kit
NADP+/NADPH Assay Kit
Synonyms
NADP+/NADPH; N/A; NADP+/NADPH Assay Kit; NADP+/NADPH assay kit
Preparation and Storage
Upon receipt, store the NADP Cycling Enzyme and NADP+ Standard at -80 degree C. Store the Colorimetric Probe at -20 degree C. Store the remaining components at 4 degree C.
Related Product Information for NADP+/NADPH assay kit
Principle of the Assay: NADP+ /NADPH Assay Kit is a convenient quantitative tool that measures NADP+ and NADPH within biological samples. The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. The intensity of the product color is proportional to the NADP+ and NADPH within a sample. A simple acid or base treatment will differentiate NADPH from NADP+ within a sample. Samples and standards are incubated for 1-4 hours and then read with a standard 96-well colorimetric plate reader (Figure 1). Samples are compared to a known concentration of NADP+ standard within the 96-well microtiter plate format.
Background/Introduction: Nicotinamide adenine dinucleotide phosphate (NADP+) is an enzymatic cofactor involved in metabolic redox and cell signaling reactions. Its main function in animal metabolism is to shift electrons from one redox reaction to another. During these reactions, the coenzyme cycles between the electron donating reduced form (NADPH) and the electron accepting oxidized form (NADP+). The major source of NADPH produced in animal cells is the oxidative branch of the pentose phosphate pathway (PPP). NADPH is involved with macromolecule biosynthesis by providing reducing power. These characteristics have made NADPH an important molecule in cancer cell proliferation and metabolism. NADPH is also involved in the accumulation of Reactive Oxygen Species (ROS) and protecting against its toxicity. It is also involved in anabolic pathways, such as fatty acid elongation and lipid and cholesterol synthesis. Understanding the metabolism of these cofactors has become important to developing new therapeutic methods against pathological disease states. NADP+/NADPH Assay Kit is a simple colorimetric assay that can measure both NADP+ and NADPH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NADP+, NADPH, and their ratio. The kit will not detect NAD or NADH. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NADP+ standards and unknown samples. The total NADP+/NADPH concentrations of unknown samples are determined by comparison with a known NADP+ standard. Determination of both NADP+ and NADPH requires two separate samples for quantification. NADP+ and NADPH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NADP+.
Background/Introduction: Nicotinamide adenine dinucleotide phosphate (NADP+) is an enzymatic cofactor involved in metabolic redox and cell signaling reactions. Its main function in animal metabolism is to shift electrons from one redox reaction to another. During these reactions, the coenzyme cycles between the electron donating reduced form (NADPH) and the electron accepting oxidized form (NADP+). The major source of NADPH produced in animal cells is the oxidative branch of the pentose phosphate pathway (PPP). NADPH is involved with macromolecule biosynthesis by providing reducing power. These characteristics have made NADPH an important molecule in cancer cell proliferation and metabolism. NADPH is also involved in the accumulation of Reactive Oxygen Species (ROS) and protecting against its toxicity. It is also involved in anabolic pathways, such as fatty acid elongation and lipid and cholesterol synthesis. Understanding the metabolism of these cofactors has become important to developing new therapeutic methods against pathological disease states. NADP+/NADPH Assay Kit is a simple colorimetric assay that can measure both NADP+ and NADPH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NADP+, NADPH, and their ratio. The kit will not detect NAD or NADH. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NADP+ standards and unknown samples. The total NADP+/NADPH concentrations of unknown samples are determined by comparison with a known NADP+ standard. Determination of both NADP+ and NADPH requires two separate samples for quantification. NADP+ and NADPH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NADP+.
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Product Notes
The NADP+/NADPH (Catalog #AAA44639) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NADP+/NADPH, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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