Application Data
OxiSelect Comet Assay Kit
OxiSelect Comet Assay Kit (3-Well Slides)
Synonyms
OxiSelect Comet; N/A; OxiSelect Comet Assay Kit (3-Well Slides); OxiSelect Comet assay kit
Assay Type
Quantitative Sandwich
Preparation and Storage
Upon receipt, store the Vista Green DNA Dye at -20 degree C. Store all other kit components at room temperature until their expiration dates.
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Related Product Information for OxiSelect Comet assay kit
Background: DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. While this counts for only a small part of the human genome's approximately 6 billion bases (3 billion base pairs), unrepaired lesions to critical genes can impede a cell's ability to carry out its function and appreciably increase the likelihood of cancer. The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic "comet tail" shape under the microscope. Extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters. The OxiSelect™ Comet Assay is a fast and sensitive kit for the measurement of cellular DNA damage. Each kit provides sufficient reagents to perform up to 75 assays.
Principle of the Assay: The OxiSelect™ Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. First, individual cells are mixed with molten agarose before application to the OxiSelect™ Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a "comet tail" shape (see Figure 1). Each kit provides sufficient reagents to perform up to 75 assays.
Principle of the Assay: The OxiSelect™ Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. First, individual cells are mixed with molten agarose before application to the OxiSelect™ Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a "comet tail" shape (see Figure 1). Each kit provides sufficient reagents to perform up to 75 assays.
Product Categories/Family for OxiSelect Comet assay kit
References
1. Ostling, O., and Johanson, K. J. (1984). Biochem. Biophys. Res. Commun. 123, 291-298.
2. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). Exp. Cell. Res. 175, 184-191.
3. Olive, P. L., Banath, J. P., and Durand, R. E. (1990). Radiat. Res. 122, 86-94.
4. De Boeck, M., Touil, N., De Visscher, G., Vande, P. A., and Kirsch-Volders, M. (2000). Mutat. Res. 469, 181-197
1. Wang, L. et al. (2014). Osmotic Stress-induced Phosphorylation of H2AX by Polo-like Kinase 3 Affects Cell Cycle Progression in Human Corneal Epithelial Cells. J Biol Chem. 289:29827-29835.
2. Savage, K.I. et al. (2014). BRCA1 Deficiency Exacerbates Estrogen-Induced DNA Damage and Genomic Instability. Cancer Res. 74:2773-2784.
3. Robin, T.P. et al. (2012). EWS/FLI1 Regulates EYA3 in Ewing Sarcoma via Modulation of miRNA-708, Resulting in Increased Cell Survival and Chemoresistance. Mol. Cancer Res. 10:1098-1108.
4. Choi, S.K. et al. (2012).Poly(ADP-Ribose) Polymerase 1 Inhibition Improves Coronary Arteriole Function in Type 2 Diabetes Mellitus. Hypertension. 59:1060-1068.
2. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). Exp. Cell. Res. 175, 184-191.
3. Olive, P. L., Banath, J. P., and Durand, R. E. (1990). Radiat. Res. 122, 86-94.
4. De Boeck, M., Touil, N., De Visscher, G., Vande, P. A., and Kirsch-Volders, M. (2000). Mutat. Res. 469, 181-197
1. Wang, L. et al. (2014). Osmotic Stress-induced Phosphorylation of H2AX by Polo-like Kinase 3 Affects Cell Cycle Progression in Human Corneal Epithelial Cells. J Biol Chem. 289:29827-29835.
2. Savage, K.I. et al. (2014). BRCA1 Deficiency Exacerbates Estrogen-Induced DNA Damage and Genomic Instability. Cancer Res. 74:2773-2784.
3. Robin, T.P. et al. (2012). EWS/FLI1 Regulates EYA3 in Ewing Sarcoma via Modulation of miRNA-708, Resulting in Increased Cell Survival and Chemoresistance. Mol. Cancer Res. 10:1098-1108.
4. Choi, S.K. et al. (2012).Poly(ADP-Ribose) Polymerase 1 Inhibition Improves Coronary Arteriole Function in Type 2 Diabetes Mellitus. Hypertension. 59:1060-1068.
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Product Notes
The OxiSelect Comet (Catalog #AAA44549) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Comet, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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