Application Data
OxiSelect Intracellular Nitric Oxide (NO) Assay Kit
OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)
Synonyms
OxiSelect Intracellular Nitric Oxide (NO); N/A; OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric); OxiSelect Intracellular Nitric Oxide (NO) assay kit
Preparation and Storage
Store the entire kit at -20 degree C. Avoid multiple freeze/thaws by aliquoting. The Nitric Oxide Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Application Data
Application Data
Application Data
Application Data
Related Product Information for OxiSelect Intracellular Nitric Oxide (NO) assay kit
Background/Introduction: Reactive nitrogen species (RNS) and reactive oxygen species (ROS) work together to damage cells and have been implicated in the pathogenesis of several disease states. RNS are a family of molecules derived from nitric oxide (NO) and superoxide anion (O2-), produced via nitric oxide synthase (NOS) and NADPH oxidase, respectively (Figure 1). Nitric oxide is an established mediator in vascular diseases, diabetes, renal ischemia, atherosclerosis, inflammatory diseases, and cancer. However, because of its extremely short half life, studies of NO and its physiological role have been challenging. The OxiSelect™ Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit's provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples.
Product Categories/Family for OxiSelect Intracellular Nitric Oxide (NO) assay kit
References
1. Novo E, and Parola M. (2008) Fibrogenesis Tissue Repair, 1:5.
2. Ignarro, LJ. (2002) J. Physiol. Pharmacol. 53:503-514.
3. Hickok, JR and Thomas, DD. (2010) Curr. Pharm. Des. 16:381-391.
4. Thomas, DD et al. (2008) Free Radic. Biol. Med. 45:18-31
2. Ignarro, LJ. (2002) J. Physiol. Pharmacol. 53:503-514.
3. Hickok, JR and Thomas, DD. (2010) Curr. Pharm. Des. 16:381-391.
4. Thomas, DD et al. (2008) Free Radic. Biol. Med. 45:18-31
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Product Notes
The OxiSelect Intracellular Nitric Oxide (NO) (Catalog #AAA44531) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Intracellular Nitric Oxide (NO), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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