Application Data
OxiSelect Superoxide Dismutase Activity Assay Kit
OxiSelect Superoxide Dismutase Activity Assay Kit
Synonyms
OxiSelect Superoxide Dismutase Activity; N/A; OxiSelect Superoxide Dismutase Activity Assay Kit; OxiSelect Superoxide Dismutase Activity assay kit
Preparation and Storage
Store kit components at -20 degree C until their expiration dates. Avoid multiple freeze/thaws by aliquoting. Chromagen Solution is light sensitive and should be maintained in amber tubes.
Application Data
Application Data
Application Data
Application Data
Related Product Information for OxiSelect Superoxide Dismutase Activity assay kit
Principle of the Assay: Superoxide anions (O2-) are generated by a Xanthine/Xanthine Oxidase (XOD) system, and then detected with a Chromagen Solution. However, in the presence of SOD, these superoxide anion concentrations are reduced, yielding less colorimetric signal.
Background/Introduction: Reactive oxygen species (ROS), such as superoxide (O2-) and hydrogen peroxide (H2O2), are constantly produced during metabolic processes in all living species. Under normal physiological conditions, cellular ROS generation is counterbalanced by the action of antioxidant enzymes and other redox molecules. However, excessive ROS accumulation will lead to cellular injury, such as damage to DNA, protein, and lipid membrane. Because of their potential harmful effects, excessive ROS must be promptly eliminated from the cells by a variety of antioxidant defense mechanisms. Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. SOD enzymes are classified into three groups: cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect™ Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions. The included chromagen produces a water-soluble formazan dye upon reduction by superoxide anions. The activity of SOD is determined as the inhibition of chromagen reduction (See Figure 1). The OxiSelect™ Superoxide Dismutase Activity Assay is a fast and reliable kit for the measurement of SOD activity from cell lysate, plasma, serum, tissue homogenates. Each Trial Size Superoxide Dismutase Activity Assay Kit provides sufficient reagents to perform up to 20 assays, including blanks, SOD standards and unknown protein samples.
Background/Introduction: Reactive oxygen species (ROS), such as superoxide (O2-) and hydrogen peroxide (H2O2), are constantly produced during metabolic processes in all living species. Under normal physiological conditions, cellular ROS generation is counterbalanced by the action of antioxidant enzymes and other redox molecules. However, excessive ROS accumulation will lead to cellular injury, such as damage to DNA, protein, and lipid membrane. Because of their potential harmful effects, excessive ROS must be promptly eliminated from the cells by a variety of antioxidant defense mechanisms. Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. SOD enzymes are classified into three groups: cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect™ Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions. The included chromagen produces a water-soluble formazan dye upon reduction by superoxide anions. The activity of SOD is determined as the inhibition of chromagen reduction (See Figure 1). The OxiSelect™ Superoxide Dismutase Activity Assay is a fast and reliable kit for the measurement of SOD activity from cell lysate, plasma, serum, tissue homogenates. Each Trial Size Superoxide Dismutase Activity Assay Kit provides sufficient reagents to perform up to 20 assays, including blanks, SOD standards and unknown protein samples.
Product Categories/Family for OxiSelect Superoxide Dismutase Activity assay kit
References
1. Lepock, J. R., Arnold, L. D., Torrie, B. H., Andrews, B., and Kruuv, J. (1985) Arch. Biochem. Biophys. 241, 243-251.2. Lepock, J. R., Frey, H. E., and Hallewell, R. A. (1990) J. Biol. Chem. 265, 21612-21618.3. Valentine, J. S., and Hart, P. J. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 3617-3622.4. Zelko, I. N., Mariani, T. J., and Folz, R. J. (2002) Free Radic. Biol. Med. 33, 337-349.
1. Alway, S. E. et al. (2015). Green tea extract attenuates muscle loss and improves muscle function during disuse, but fails to improve muscle recovery following unloading in aged rats. J. Appl Physiol (1985). 118:319-330. 2. Rajapaksha, A. et al. (2015). Effects of macromolecular crowding on the structure of a protein complex: a small-angle scattering study of superoxide dismutase. Biophys J. 108:967-974. 3. Medlow, P. et al. (2015). Exercise training protects the LDL I subfraction from oxidation susceptibility in an aged human population. Atherosclerosis. 239:516-522.4. Akgüllü, Ç. et al. (2015). The usefulness of carvedilol and nebivolol in preventing contrast nephropathy in rats. Ren Fail. doi:10.3109/0886022X.2015.1006087. 5. Kim, S. et al. (2015). Ameliorating the effect of astragaloside IV on learning and memory deficit after chronic cerebral hypoperfusion in rats. Molecules. 20:1904-1921.6. Mohammad, M. K. et al. (2014). Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose X-ray in mice. Biomed Res Int. 2014:512834.7. Mohamed, M. I. et al. (2014). Induction of oxidative stress following low dose ionizing radiation in ICR mice. World J Med Sci. 10:198-203. 8. Yang, X. et al. (2014). Exposure to excess insulin (glargine) induces type 2 diabetes mellitus in mice fed on a chow diet. J Endocrinol. 221:469-480.9. Wysocki, J. et al. (2014). ACE2 deficiency increases NADPH-mediated oxidative stress in the kidney. Physiol. Reports 2:e00264.10. Yin, J. et al. (2014). Development of an antioxidant system after early weaning in piglets. J. Anim. Sci. 92:612-619.11. Liu, Z.Z. et al. (2014). Iodinated contrast media cause direct tubular cell damage, leading to oxidative stress, low nitric oxide, and impairment of tubuloglomerular feedback. Am. J. Physiol. Renal Physiol. 306:F864-F872.12. Palipoch, S. et al. (2013). Amelioration of cisplatin-induced nephrotoxicity in rats by curcumin and alpha-tocopherol. Tropical Journal of Pharmaceutical Research. 12:973-979.13. Gong, E.J. et al. (2013). Low-dose-rate radiation exposure leads to testicular damage with decreases in DNMT1 and HDAC1 in the murine testis. J Radiat Res. 10.1093/jrr/rrt090.14. Song, J. et al. (2013). Nicotinamide phosphoribosyltransferase is required for the calorie restriction-mediated improvements in oxidative stress, mitochondrial biogenesis, and metabolic adaptation. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glt122.15. Paneni, F. et al. (2013). Deletion of the activated protein-1 transcription factor JunD induces oxidative stress and accelerates age-related endothelial dysfunction. Circulation. 127:1229-1240.16. Zhang, Z. et al. (2012). TRPM2 Ca2+ channel regulates energy balance and glucose metabolism. Am J Physiol Endocrinol Metab. 302:E807-E816.17. Connell, B.J. et al. (2012). UPEI-100, a conjugate of lipoic acid and apocynin, mediates neuroprotection in a rat model of ischemia/reperfusion. Am J Physiol Regulatory Integrative Comp Physiol. 302:R866-R895.
1. Alway, S. E. et al. (2015). Green tea extract attenuates muscle loss and improves muscle function during disuse, but fails to improve muscle recovery following unloading in aged rats. J. Appl Physiol (1985). 118:319-330. 2. Rajapaksha, A. et al. (2015). Effects of macromolecular crowding on the structure of a protein complex: a small-angle scattering study of superoxide dismutase. Biophys J. 108:967-974. 3. Medlow, P. et al. (2015). Exercise training protects the LDL I subfraction from oxidation susceptibility in an aged human population. Atherosclerosis. 239:516-522.4. Akgüllü, Ç. et al. (2015). The usefulness of carvedilol and nebivolol in preventing contrast nephropathy in rats. Ren Fail. doi:10.3109/0886022X.2015.1006087. 5. Kim, S. et al. (2015). Ameliorating the effect of astragaloside IV on learning and memory deficit after chronic cerebral hypoperfusion in rats. Molecules. 20:1904-1921.6. Mohammad, M. K. et al. (2014). Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose X-ray in mice. Biomed Res Int. 2014:512834.7. Mohamed, M. I. et al. (2014). Induction of oxidative stress following low dose ionizing radiation in ICR mice. World J Med Sci. 10:198-203. 8. Yang, X. et al. (2014). Exposure to excess insulin (glargine) induces type 2 diabetes mellitus in mice fed on a chow diet. J Endocrinol. 221:469-480.9. Wysocki, J. et al. (2014). ACE2 deficiency increases NADPH-mediated oxidative stress in the kidney. Physiol. Reports 2:e00264.10. Yin, J. et al. (2014). Development of an antioxidant system after early weaning in piglets. J. Anim. Sci. 92:612-619.11. Liu, Z.Z. et al. (2014). Iodinated contrast media cause direct tubular cell damage, leading to oxidative stress, low nitric oxide, and impairment of tubuloglomerular feedback. Am. J. Physiol. Renal Physiol. 306:F864-F872.12. Palipoch, S. et al. (2013). Amelioration of cisplatin-induced nephrotoxicity in rats by curcumin and alpha-tocopherol. Tropical Journal of Pharmaceutical Research. 12:973-979.13. Gong, E.J. et al. (2013). Low-dose-rate radiation exposure leads to testicular damage with decreases in DNMT1 and HDAC1 in the murine testis. J Radiat Res. 10.1093/jrr/rrt090.14. Song, J. et al. (2013). Nicotinamide phosphoribosyltransferase is required for the calorie restriction-mediated improvements in oxidative stress, mitochondrial biogenesis, and metabolic adaptation. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glt122.15. Paneni, F. et al. (2013). Deletion of the activated protein-1 transcription factor JunD induces oxidative stress and accelerates age-related endothelial dysfunction. Circulation. 127:1229-1240.16. Zhang, Z. et al. (2012). TRPM2 Ca2+ channel regulates energy balance and glucose metabolism. Am J Physiol Endocrinol Metab. 302:E807-E816.17. Connell, B.J. et al. (2012). UPEI-100, a conjugate of lipoic acid and apocynin, mediates neuroprotection in a rat model of ischemia/reperfusion. Am J Physiol Regulatory Integrative Comp Physiol. 302:R866-R895.
Similar Products
Product Notes
The OxiSelect Superoxide Dismutase Activity (Catalog #AAA44593) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Superoxide Dismutase Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.