Phosphate Assay Kit
Phosphate Assay Kit
Synonyms
Phosphate; N/A; Phosphate Assay Kit; Phosphate assay kit
Sequence Length
185
Preparation and Storage
Store at 2-8 degree C. Valid for 12 months
Related Product Information for Phosphate assay kit
Principle of the Assay: In aqueous solution, nitric oxide rapidly degrades to nitrate and nitrite. Spectrophotometric quantitation of nitrite using Griess Reagent is straightforward, but does not measure nitrate. This kit employs metallic cadmium for quantitative conversion of nitrate to nitrite (Figure 1) prior to quantitation of nitrate using Griess reagent — thus providing for accurate determination of total NO production. This kit can be used to accurately measure as little as 1 pmol/mL (~1uM) NO produced in aqueous solutions. Very little sample is required (1 to 100uL depending on the [NO] in the sample). The completed reaction is read at 540 nm!!Background/Introduction: This kit allows you to measure total nitric oxide (NO) produced for in vitro experimental systems following conversion of nitrate to nitrite by metallic cadmium. Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem involves the conversion of nitrate to nitrite catalyzed by cadmium. This is then followed by the quantitation of nitrite using the Griess reaction. This kit is suitable for the quantitative determination of total nitric oxide in samples that have high protein concentrations. The cadmium catalyst proves far more robust when in the presence of harsh deproteinating reagents than the alternative, nitrate reductase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
phosphate, partial
Similar Products
Product Notes
The Phosphate (Catalog #AAA172737) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Phosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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