Superoxide Anion Scavenging Capacity Assay Kit
Superoxide Anion Scavenging Capacity Assay Kit
Synonyms
Superoxide Anion Scavenging Capacity; N/A; Superoxide Anion Scavenging Capacity Assay Kit; Superoxide Anion Scavenging Capacity assay kit
Samples
Animal and Plant Tissues, Cells, Blood, Drug
Application Notes
Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2?) is provided by xanthine oxidase (XO) catalyzed reaction. O2? reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2? thus less O2? is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm.
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Related Product Information for Superoxide Anion Scavenging Capacity assay kit
Principle of the Assay: The superoxide anion (O2-) is a short-lived radical production by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and -radiation, or derived from oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components and causes damage to lipids, proteins and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. The study of scavenging superoxide anion radicals has been gained more and more attention. CheKine™ Micro Superoxide Anion Scavenging Capacity Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in animal and plant tissues, cell and other biological fluids. In this assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified. Sample scavenges the O2- thus less O2- is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm.
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Product Notes
The Superoxide Anion Scavenging Capacity (Catalog #AAA31526) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2?) is provided by xanthine oxidase (XO) catalyzed reaction. O2? reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2? thus less O2? is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm. Researchers should empirically determine the suitability of the Superoxide Anion Scavenging Capacity for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Anion Scavenging Capacity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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