Standard Curve (Sample)
Superoxide Dismutases (SOD) Assay Kit | SOD assay kit
Superoxide Dismutases (SOD) Assay Kit
Synonyms
Superoxide Dismutases (SOD); N/A; Superoxide Dismutases (SOD) Assay Kit; SOD assay kit
Sequence Length
142
Samples
Serem, Plasma, Animal and Plant Tissues, Cells
Application Notes
Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2?) is provided by xanthine oxidase (XO) catalyzed reaction. O2? reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2? thus less O2? is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm.
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SOD assay kit
Principle of the Assay: Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide anion into O2 and H2O2. They are an important antioxidant defense against the toxicity of superoxide radical in all cells exposed to O2. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Three forms of superoxide dismutase are present in humans. SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. CheKine™ Micro Superoxide Dismutases (SOD) Activity Assay Kit provides a simple and easy assay for the quantitative determination of SOD enzyme activity in serum, plasma, animal and plant tissues, cells lysates and other biological fluids. In the assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm. The activity of SOD was negatively correlated with the amount of formazan.
Product Categories/Family for SOD assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
SOD, partial
Similar Products
Product Notes
The SOD (Catalog #AAA31523) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2?) is provided by xanthine oxidase (XO) catalyzed reaction. O2? reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2? thus less O2? is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm. Researchers should empirically determine the suitability of the SOD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutases (SOD), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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