Standard Curve (Sample)
The total protein Assay Kit
The total protein assay kit (with standard : BCA method)
Standard Curve (Sample)
Standard Curve (Sample)
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Product Notes
The The total protein (Catalog #AAA22074) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "The total protein, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What are protein assays?
Protein assays are quantitative techniques that measure total protein concentration in samples using colorimetric or fluorometric detection methods. BCA assays use copper ion reduction followed by bicinchoninic acid chelation for sensitive protein quantification.
What is the BCA protein assay kit?
The BCA (Bicinchoninic Acid) protein assay kit is a colorimetric assay based on copper ion reduction by proteins in alkaline conditions, forming a purple complex detectable at 562 nm for accurate protein concentration measurement.
What is the detection range and sensitivity of the protein assay?
BCA assay detection ranges from 20–2,000 µg/mL with standard protocols, or 125–2,000 µg/mL for microplate formats. Enhanced protocols shift the range to 5–250 µg/mL for more dilute samples with improved sensitivity.
Does the kit work with detergents or reducing agents?
Standard BCA assays are compatible with most ionic and non-ionic detergents but incompatible with reducing agents like DTT. However, reducing agent-compatible versions are available with a compatibility reagent allowing up to 5 mM DTT.
How long does the protein assay take to complete?
The rapid BCA assay completes in 5 minutes at room temperature, while standard protocols require 30 minutes at 37°C or 2 hours at room temperature, depending on the kit formulation and incubation conditions.
What storage conditions are required for the reagents?
BCA assay reagents are typically stored at room temperature with light protection for 12 months. Reconstituted compatibility reagent, if used, remains stable for 8 hours at 4°C when protected from light.
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