Samples
Serum, Plasma, Animal and Plant Tissues, Cells, Bacteria, Urine
Application Notes
Total Antioxidant Capacity (TAC) Assay Kit provides a simple and easy colorimetric assay for measuring Total Antioxidant Capacity in serum, plasma, cell culture supernatants, urine, tissue/ cell lysates and other biological fluids. In this assay, Fe3+-TPTZ is reduced by antioxidant to Fe2+-TPTZ. The enzyme catalyzed reaction products Fe2+-TPTZ can be measured at a colorimetric readout at 593 nm.
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Related Product Information for TAC assay kit
Principle of the Assay: Antioxidants play an important role in preventing the formation and scavenging of free radicals and other potentially toxic oxidizingspecies. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). As oxidative stress contributes tothe development of many diseases including Alzheimer's disease, Parkinson's disease, diabetes, rheumatoid arthritis andneurodegeneration, the use of antioxidants in pharmacology is intensively studied. CheKine™ Micro Total Antioxidant Capacity(TAC) Assay Kit provides a convenient tool for detection of Total Antioxidant Capacity (TAC) in serum, plasma, animal and planttissues, cells, bacteria, urine and other biological fluids. The principle is that in an acidic environment, antioxidants can reduceFe3+-Tripyridine triazine (Fe3+-TPTZ) to produce blue Fe2+-TPTZ, and the total antioxidant capacity of samples was obtained bydetermining the content of Fe2+-TPTZ at 593 nm.
Product Categories/Family for TAC assay kit
Similar Products
Product Notes
The TAC (Catalog #AAA31528) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Total Antioxidant Capacity (TAC) Assay Kit provides a simple and easy colorimetric assay for measuring Total Antioxidant Capacity in serum, plasma, cell culture supernatants, urine, tissue/ cell lysates and other biological fluids. In this assay, Fe3+-TPTZ is reduced by antioxidant to Fe2+-TPTZ. The enzyme catalyzed reaction products Fe2+-TPTZ can be measured at a colorimetric readout at 593 nm. Researchers should empirically determine the suitability of the TAC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Total Antioxidant Capacity (TAC), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.