Total Phosphatidic Acid Assay Kit
Total Phosphatidic Acid Assay Kit (Fluorometric)
Synonyms
Total Phosphatidic Acid; N/A; Total Phosphatidic Acid Assay Kit (Fluorometric); Total Phosphatidic Acid assay kit
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store the entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Related Product Information for Total Phosphatidic Acid assay kit
Background/Introduction: Phosphatidic Acid (PA) is a critical precursor for the biosynthesis of many lipids in the cell. PA plays a critical role in membrane structure and acts as a signaling lipid, recruiting cytosolic proteins to the cell membrane. Within the cell, PA concentrations are maintained at extremely low levels by the activity of potent phospholipid phosphatases, converting phosphatidic acid to diacylglycerol (DAG). Because DAG is another important lipid precursor, it too is rapidly metabolised into other membrane lipid components (e.g. phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine). Ultimately, measurement of cellular PA is useful for monitoring lipid synthesis and metabolism. Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content, including lysophosphatidic acid (LPA), in samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid in samples to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit's Fluorometric Probe (Ex. 530-560 nm/Em. 585-595 nm). The Total Phosphatidic Acid Assay Kit is a simple, fluorometric assay that quantitatively measures total PA (PA and LPA) in samples using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains an L-alpha-Phosphatidic Acid Standard and has a detection sensitivity limit of ~5 uM.
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Product Notes
The Total Phosphatidic Acid (Catalog #AAA44640) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Phosphatidic Acid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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