Human Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit | AT-1 elisa kit

Human Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit

Cat. Num. AAA16102

• Reactivity: Human
• Synonyms: Angiotensin 2 Receptor type 1 Autoantibodies; N/A; Human Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit; Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit (Human); AT-1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A2R1Ab. No significant cross-reactivity or interference between A2R1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between A2R1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for AT-1 elisa kit

Principle of the Assay: A2R1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a specific antigen and an A2R1Ab-HRP conjugate. The assay sample and buffer are incubated together with A2R1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A2R1Ab concentration since A2R1Ab from samples and A2R1Ab-HRP conjugate compete for the a specific antigen binding site. Since the number of sites is limited, as more sites are occupied by A2R1Ab from the sample, fewer sites are left to bind A2R1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Ab concentration in each sample is interpolated from this standard curve.

Intended Uses: This A2R1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human A2R1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

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Human ELISA Kit