Human Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit | AT-1 elisa kit
Human Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit
Reactivity
Human
Synonyms
Angiotensin 2 Receptor type 1 Autoantibodies; N/A; Human Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit; Angiotensin 2 Receptor type 1 Autoantibodies ELISA Kit (Human); AT-1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of A2R1Ab. No significant cross-reactivity or interference between A2R1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between A2R1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Related Product Information for AT-1 elisa kit
Principle of the Assay: A2R1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a specific antigen and an A2R1Ab-HRP conjugate. The assay sample and buffer are incubated together with A2R1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A2R1Ab concentration since A2R1Ab from samples and A2R1Ab-HRP conjugate compete for the a specific antigen binding site. Since the number of sites is limited, as more sites are occupied by A2R1Ab from the sample, fewer sites are left to bind A2R1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Ab concentration in each sample is interpolated from this standard curve.
Intended Uses: This A2R1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human A2R1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This A2R1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human A2R1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for AT-1 elisa kit
