Standard Curve (Sample)
(Typical Standard Curve for ATP5a ELISA (Sandwich))
Human ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide ELISA Kit | ATP5a elisa kit
Human ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a) (Sandwich ELISA) ELISA Kit
Gene Names
ATP5A1; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2
Reactivity
Human
Synonyms
ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide; N/A; Human ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a) (Sandwich ELISA) ELISA Kit; ATP5A1; ATP5AL2; ATPM; OMR; ORM; hATP1; ELISA Kit for ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a); ATP5a elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a).
No significant cross-reactivity or interference between ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a) and analogues was observed. Reactive with Human ATP5a / ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide
No significant cross-reactivity or interference between ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a) and analogues was observed. Reactive with Human ATP5a / ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.115ng/mL
Detection Method
Colormetric
Assay Precision
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year
Standard Curve (Sample)
(Typical Standard Curve for ATP5a ELISA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for ATP5a ELISA (Sandwich))
Related Product Information for ATP5a elisa kit
Principle of the Assay||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide (ATP5a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ATP5a elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,751 Da
NCBI Official Full Name
ATP synthase subunit alpha, mitochondrial isoform c
NCBI Official Synonym Full Names
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
NCBI Official Symbol
ATP5A1
NCBI Official Synonym Symbols
OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2
NCBI Protein Information
ATP synthase subunit alpha, mitochondrial; ATP sythase (F1-ATPase) alpha subunit; ATP synthase alpha chain, mitochondrial; mitochondrial ATP synthetase, oligomycin-resistant; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform
UniProt Protein Name
ATP synthase subunit alpha, mitochondrial
UniProt Gene Name
ATP5A1
UniProt Synonym Gene Names
ATP5A; ATP5AL2; ATPM
UniProt Entry Name
ATPA_HUMAN
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Product Notes
The Human ATP5a atp5a1 (Catalog #AAA190044) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190044 ELISA Kit recognizes Human ATP5a. It is sometimes possible for the material contained within the vial of "ATPase, H+ Transporting, Mitochondrial F1 Complex Alpha Polypeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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