Human Ataxin 2 ELISA Kit | ATXN2 elisa kit

Human Ataxin 2 ELISA Kit

Cat. Num. AAA16299

• Gene Names: ATXN2; ATX2; SCA2; ASL13; TNRC13
• Reactivity: Human
• Synonyms: Ataxin 2; N/A; Human Ataxin 2 ELISA Kit; ATXN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATXN2. No significant cross-reactivity or interference between ATXN2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATXN2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Intended Uses: This ATXN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATXN2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ATXN2 elisa kit

Principle of the Assay: ATXN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATXN2 antibody and an ATXN2-HRP conjugate. The assay sample and buffer are incubated together with ATXN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATXN2 concentration since ATXN2 from samples and ATXN2-HRP conjugate compete for the anti-ATXN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATXN2 from the sample, fewer sites are left to bind ATXN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATXN2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ATXN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATXN2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for ATXN2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 171543895
• NCBI GeneID: 6311
• NCBI Accession #: NP_002964.3
• NCBI GenBank Nucleotide #: NM_002973.3
• Molecular Weight: 132,884 Da
• NCBI Official Full Name: ataxin-2
• NCBI Official Synonym Full Names: ataxin 2
• NCBI Official Symbol: ATXN2
• NCBI Official Synonym Symbols: ATX2; SCA2; ASL13; TNRC13
• NCBI Protein Information: ataxin-2; spinocerebellar ataxia type 2 protein; trinucleotide repeat-containing gene 13 protein
• UniProt Protein Name: Ataxin-2
• UniProt Gene Name: ATXN2
• UniProt Synonym Gene Names: ATX2; SCA2; TNRC13
• UniProt Entry Name: ATX2_HUMAN