Standard Curve (Sample)
Mouse azurocidin ELISA Kit | AZU elisa kit
Mouse azurocidin ELISA Kit
Gene Names
AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
Reactivity
Mouse
Synonyms
azurocidin; N/A; Mouse azurocidin ELISA Kit; azurocidin (Mouse); AZU elisa kit
Reactivity
Mouse
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AZU elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This AZU ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AZU in the sample, this AZU ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AZU concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AZU utilizes a multiclonal anti-AZU antibody and an AZU-HRP conjugate. The assay sample and buffer are incubated together with AZU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AZU concentration since AZU from samples and AZU-HRP conjugate compete for the anti-AZU antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU from the sample, fewer sites are left to bind AZU-HRP conjugate. Standards of known AZU concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AZU. The AZU concentration in each sample is interpolated from this standard curve.
Intended Uses: This AZU ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AZU in the sample, this AZU ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AZU concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AZU utilizes a multiclonal anti-AZU antibody and an AZU-HRP conjugate. The assay sample and buffer are incubated together with AZU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AZU concentration since AZU from samples and AZU-HRP conjugate compete for the anti-AZU antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU from the sample, fewer sites are left to bind AZU-HRP conjugate. Standards of known AZU concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AZU. The AZU concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AZU elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,886 Da
NCBI Official Full Name
azurocidin preproprotein
NCBI Official Synonym Full Names
azurocidin 1
NCBI Official Symbol
AZU1
NCBI Official Synonym Symbols
AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
NCBI Protein Information
azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37
UniProt Protein Name
Azurocidin
UniProt Gene Name
AZU1
UniProt Entry Name
CAP7_HUMAN
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Product Notes
The Mouse AZU azu1 (Catalog #AAA81881) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA81881 ELISA Kit recognizes Mouse AZU. It is sometimes possible for the material contained within the vial of "azurocidin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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