Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 ?g/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 4.552-5.720 ng/mL.)
Desmoglein-3 (DSG3) Active Protein | DSG3 active protein
Recombinant Human Desmoglein-3 (DSG3), partial (Active)
Gene Names
DSG3; PVA; CDHF6
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Desmoglein-3 (DSG3); N/A; Recombinant Human Desmoglein-3 (DSG3), partial (Active); 130 kDa pemphigus vulgaris antigen; PVA; Cadherin family member 6; DSG3 active protein
Host
Baculovirus
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Sequence Positions
50-615aa; Partial
Sequence
EWVKFAKPCREGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFVVDKNTGDINITAIVDREETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASNSLVMILNATDADEPNHLNSKIAFKIVSQEPAGTPMFLLSRNTGEVRTLTNSLDREQASSYRLVVSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQVTDLDEEYTDNWLAVYFFTSGNEGNWFEIQTDPRTNEGILKVVKALDYEQLQSVKLSIAVKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPDFNDNCPTAVLEKDAVCSSSPSVVVSARTLNNRYTGPYTFALEDQPVKLPAVWSITTLNATSALLRAQEQIPPGVYHISLVLTDSQNNRCEMPRSLTLEVCQCDNRGICGTSYPTTSPGTRYGRPHSGR$
Species
Homo sapiens (Human)
Tag
C-terminal 6xHis-tagged
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Research Area
Others
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 ug/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 4.552-5.720 ng/mL.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 ?g/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 4.552-5.720 ng/mL.)
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 ?g/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 4.552-5.720 ng/mL.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Related Product Information for DSG3 active protein
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Product Categories/Family for DSG3 active protein
References
"Expression and regulation of cornified envelope proteins in human corneal epithelium."Tong L., Corrales R.M., Chen Z., Villarreal A.L., De Paiva C.S., Beuerman R., Li D.Q., Pflugfelder S.C.Invest Ophthalmol Vis Sci 47:1938-1946(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
107,533 Da
NCBI Official Full Name
Desmoglein-3
NCBI Official Synonym Full Names
desmoglein 3
NCBI Official Symbol
DSG3
NCBI Official Synonym Symbols
PVA; CDHF6
NCBI Protein Information
desmoglein-3; cadherin family member 6; pemphigus vulgaris antigen; 130-kD pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen
UniProt Protein Name
Desmoglein-3
UniProt Gene Name
DSG3
UniProt Synonym Gene Names
CDHF6; PVA
UniProt Entry Name
DSG3_HUMAN
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Product Notes
The DSG3 dsg3 (Catalog #AAA279209) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 50-615aa; Partial. The amino acid sequence is listed below: EWVKFAKPCR EGEDNSKRNP IAKITSDYQA TQKITYRISG VGIDQPPFGI FVVDKNTGDI NITAIVDREE TPSFLITCRA LNAQGLDVEK PLILTVKILD INDNPPVFSQ QIFMGEIEEN SASNSLVMIL NATDADEPNH LNSKIAFKIV SQEPAGTPMF LLSRNTGEVR TLTNSLDREQ ASSYRLVVSG ADKDGEGLST QCECNIKVKD VNDNFPMFRD SQYSARIEEN ILSSELLRFQ VTDLDEEYTD NWLAVYFFTS GNEGNWFEIQ TDPRTNEGIL KVVKALDYEQ LQSVKLSIAV KNKAEFHQSV ISRYRVQSTP VTIQVINVRE GIAFRPASKT FTVQKGISSK KLVDYILGTY QAIDEDTNKA ASNVKYVMGR NDGGYLMIDS KTAEIKFVKN MNRDSTFIVN KTITAEVLAI DEYTGKTSTG TVYVRVPDFN DNCPTAVLEK DAVCSSSPSV VVSARTLNNR YTGPYTFALE DQPVKLPAVW SITTLNATSA LLRAQEQIPP GVYHISLVLT DSQNNRCEMP RSLTLEVCQC DNRGICGTSY PTTSPGTRYG RPHSGR$. It is sometimes possible for the material contained within the vial of "Desmoglein-3 (DSG3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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