Bioactivity
(Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
Erythropoietin/EPO Active Protein | EPO active protein
Recombinant Mouse Erythropoietin/EPO Protein
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
Erythropoietin/EPO; N/A; Recombinant Mouse Erythropoietin/EPO Protein; Erythropoietin; erythropoietin isoform 1 precursor; Epo; EPO active protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
27-192aa
Sequence
Applicable Applications for EPO active protein
Bioactivity, SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
His-Tag
Predicted N-term
Ala
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range <= 2ng/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
Bioactivity
(Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for EPO active protein
EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant mouse EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for EPO active protein
References
Koury, M.J., et al. (2005) Exp. Hematol. 33:1263. Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
19.8kDa (176aa)
UniProt Protein Name
Erythropoietin
UniProt Gene Name
Epo
UniProt Entry Name
EPO_MOUSE
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Product Notes
The EPO epo (Catalog #AAA48368) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 27-192aa. AAA Biotech's Erythropoietin/EPO can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the EPO epo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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