Flt3 ligand/FLT3LG Active Protein | FLT3LG active protein

Recombinant human Flt3 ligand/FLT3LG protein

Cat. Num. AAA49008

• Gene Names: FLT3LG; FL; FLG3L; FLT3L
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Flt3 ligand/FLT3LG; N/A; Recombinant human Flt3 ligand/FLT3LG protein; Fms-related tyrosine kinase 3 ligand isoform 1; Fms-related tyrosine kinase 3 ligand; FLT3LG; FL; FLG3L; FLT3L; IMD125; Flt3 ligand; SL cytokine; FLT3LG active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-184aa
• Sequence: Thr 27; TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP

• Applicable Applications for FLT3LG active protein: Bioactivity, SDS-PAGE
• Species: Human
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Related Product Information for FLT3LG active protein

FLT3LG, also known as fms-related tyrosine kinase 3 ligand, is an alpha-cytokine that promotes the differentiation of multiple hematopoietic cell lineages. This protein acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It is crucial for steady-state pDC and cDC development. It controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Additionally, FLT3LG synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation. Animal studies show that it reduces the severity of experimentally induced allergic inflammation. Recombinant human FLT3LG, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for FLT3LG active protein

Angiogenesis; Proteins

References

Klein O., et al. (2013) Eur. J. Immunol. 43(2):533-539.
Nakamori Y., et al. (2012) Br. J. Haematol. 157(6):674-686.

NCBI and Uniprot Product Information

• NCBI GI #: 38455416
• NCBI GeneID: 2323
• NCBI Accession #: NP_001450
• NCBI GenBank Nucleotide #: NM_001459.3
• UniProt Accession #: P49771; Q05C96; A0AVC2; B9EGH2
• Molecular Weight: 19.0kDa (166aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: fms-related tyrosine kinase 3 ligand isoform 1
• NCBI Official Synonym Full Names: fms related tyrosine kinase 3 ligand
• NCBI Official Symbol: FLT3LG
• NCBI Official Synonym Symbols: FL; FLG3L; FLT3L
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• UniProt Gene Name: FLT3LG
• UniProt Synonym Gene Names: Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_HUMAN