Bioactivity
(Mouse IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ? 0.15 ng/ml.)
IL-1 alpha/IL-1F1 Active Protein | IL-1 alpha active protein
Recombinant mouse IL-1 alpha /IL-1F1 protein
Gene Names
Il1a; Il-1a
Applications
SDS-Page
Purity
> 90% by SDS - PAGE
Synonyms
IL-1 alpha/IL-1F1; N/A; Recombinant mouse IL-1 alpha /IL-1F1 protein; Il1a; Il-1a; Interleukin-1 alpha; IL-1 alpha; IL-1F1; IL-1 alpha active protein
Host
Baculovirus
Purity/Purification
> 90% by SDS - PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
115-270aa
Sequence
SAPYTYQ SDLRYKLMKL VRQKFVMNDS LNQTIYQDVD KHYLSTTWLN DLQQEVKFDM YAYSSGGDDS KYPVTLKISD SQLFVSAQGE DQPVLLKELP ETPKLITGSE TDLIFFWKSI NSKNYFTSAA YPELFIATKE QSRVHLARGL PSMTDFQIS
Sequence Length
270
Applicable Applications for IL-1 alpha active protein
Bioactivity, SDS-PAGE
Antigen Species
Mouse
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Predicted Nterm
Ala
Tag Information
His-Tag
Biological Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ? 0.15 ng/ml.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Mouse IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ? 0.15 ng/ml.)
Bioactivity
(Mouse IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ? 0.15 ng/ml.)
SDS-PAGE
(SDS-Page)
SDS-PAGE
(SDS-Page)
Related Product Information for IL-1 alpha active protein
IL-1 alpha /IL-1F1, also known as interleukin-1 alpha, is a member of the interleukin-1 cytokine family. Cytokines are protein signaling compounds that are the main mediators of the immune response. This protein is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Also, it stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation. Recombinant mouse IL-1 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for IL-1 alpha active protein
References
Dinarello CA., et al. (1997) Semin Oncol. 24:S9-81-S9-93.;; Bankers-Fulbright JL., et al. (1996) Life Sci. 59:61-83.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19.0kDa (165aa)
NCBI Official Full Name
interleukin-1 alpha
NCBI Official Synonym Full Names
interleukin 1 alpha
NCBI Official Symbol
Il1a
NCBI Official Synonym Symbols
Il-1a
NCBI Protein Information
interleukin-1 alpha
UniProt Protein Name
Interleukin-1 alpha
UniProt Gene Name
Il1a
UniProt Synonym Gene Names
IL-1 alpha
UniProt Entry Name
IL1A_MOUSE
Similar Products
Product Notes
The IL-1 alpha il1a (Catalog #AAA48354) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 115-270aa with tag His-Tag!!Biological Activity||Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ? 0.15 ng/ml. AAA Biotech's IL-1 alpha/IL-1F1 can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the IL-1 alpha il1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SAPYTYQ SDLRYKLMKL VRQKFVMNDS LNQTIYQDVD KHYLSTTWLN DLQQEVKFDM YAYSSGGDDS KYPVTLKISD SQLFVSAQGE DQPVLLKELP ETPKLITGSE TDLIFFWKSI NSKNYFTSAA YPELFIATKE QSRVHLARGL PSMTDFQIS. It is sometimes possible for the material contained within the vial of "IL-1 alpha/IL-1F1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.