Bioactivity
(HTRF Assay for Recombinant MINK1 (1-320) activity. 1 uM STK S3 substrate was incubated with different concentrations of MINK1 (1-320) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
MINK1 active protein
Recombinant MINK1 (1-320) protein
Purity
>95%
Synonyms
MINK1; N/A; Recombinant MINK1 (1-320) protein; Misshapen Like Kinase 1; MINK1 active protein
Host
Baculovirus
Purity/Purification
>95%
Form/Format
Recombinant MINK1 (1-320) protein is supplied at a concentration of 0.25 ug/ul in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Protein Details
Recombinant MINK1 (1-320) protein that includes amino acids 1-320 of human MINK1 protein (accession number NP_722549.2) was expressed in a baculovirus expression system and contains an N-terminal DYKDDDDK tag. The molecular weight of the protein is 37.7 kDa.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Bioactivity
(HTRF Assay for Recombinant MINK1 (1-320) activity. 1 uM STK S3 substrate was incubated with different concentrations of MINK1 (1-320) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Bioactivity
(HTRF Assay for Recombinant MINK1 (1-320) activity. 1 uM STK S3 substrate was incubated with different concentrations of MINK1 (1-320) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant MINK1 (1-320) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 37.7 kDa Purity: >95%)
SDS-PAGE
(Recombinant MINK1 (1-320) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 37.7 kDa Purity: >95%)
Related Product Information for MINK1 active protein
MINK1 (Misshapen Like Kinase 1) is a serine/threonine kinase which acts as a negative regulator of Rasrelated Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. MINK1 is required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. MINK1 can activate the JNK and MAPK14/p38 pathways and mediate stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Diseases associated with MINK1 include Alzheimer Disease 15 and Shipyard Eye.
Product Categories/Family for MINK1 active protein
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Product Notes
The MINK1 (Catalog #AAA60330) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MINK1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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