OX40 Ligand/TNFSF4 Active Protein | OX40 active protein

Recombinant mouse OX40 Ligand/TNFSF4 Protein

Cat. Num. AAA48397

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: OX40 Ligand/TNFSF4; N/A; Recombinant mouse OX40 Ligand/TNFSF4 Protein; gp3; OX4;CD252; OX40 ligand; Ath-1; Ath1; CD134L; gp34; OX-40L; Ox40l; Tnlg2b; TXGP1; Txgp1l; tumor necrosis factor ligand superfamily member 4; OX40 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol.
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 51-198aa
• Sequence: SSSPAKD PPIQRLRGAV TRCEDGQLFI SSYKNEYQTM EVQNNSVVIK CDGLYIIYLK GSFFQEVKID LHFREDHNPI SIPMLNDGRR IVFTVVASLA FKDKVYLTVN APDTLCEHLQ INDGELIVVQ LTPGYCAPEG SYHSTVNQVP L

• Applicable Applications for OX40 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured by its binding ability in a functional ELISA with Human TNFRSF4.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Mouse TNFSF4 is coated at 1 ug/ml (100 ul/well) can bind Human TNFRSF4 (CAT# ATGP3686) in a Functional ELISA assay.)

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stainLane 1 : reducing conditionLane 2 : non-reducing condition)

Related Product Information for OX40 active protein

OX40 Ligand, also known as Inositol monophosphatase 3, is a member of the tumor necrosis factor (TNF) receptor family. It is expressed on many antigen-presenting cells such as DC2s, macrophages, and activated B lymphocytes. The receptor for OX40L is OX40 that is expressed predominantly on activated CD4+ T cells. Expression of OX40 is transient following engagement of T cell receptors. The ligation of OX40-OX40L is a source of survival signal for T cells and enables the development of memory T cells. Also, It stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion. OX40-OX40 Ligand signaling is involved in graft-versus-host disease and autoimmune disease. Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease. Recombinant mouse OX40 Ligand, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for OX40 active protein

Immunology; Proteins

References

Damayanti T., et al, (2010) Am. J. Respir. Crit. Care Med. 181(7):688-698. Ishii N., et al, (2010) Adv. Immunol. 105:63-98.

NCBI and Uniprot Product Information

• UniProt Accession #: P43488
• Molecular Weight: 17.7kDa (157aa)
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• UniProt Gene Name: Tnfsf4
• UniProt Synonym Gene Names: Ox40l; Txgp1l; OX40L
• UniProt Entry Name: TNFL4_MOUSE