Bioactivity
(HTRF assay for RPS6KA2/RSK3 protein activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA2/RSK3 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
RPS6KA2/RSK3 active protein
Recombinant RPS6KA2/RSK3 protein
Purity
>=90%
Synonyms
RPS6KA2/RSK3; N/A; Recombinant RPS6KA2/RSK3 protein; ribosomal S6 kinase; RPS6KA2/RSK3 active protein
Host
Baculovirus
Purity/Purification
>=90%
Form/Format
Recombinant RPS6KA2/RSK3 protein is supplied at a concentration in 25 mM HEPES, NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Protein Details
Recombinant RPS6KA2/RSK3 protein was expressed in baculovirus expression system as the full-length protein (accession number NP_066958.2) with a N-terminal DYKDDDDK tag. The molecular weight of the protein is 84.5 kDa.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Bioactivity
(HTRF assay for RPS6KA2/RSK3 protein activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA2/RSK3 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Bioactivity
(HTRF assay for RPS6KA2/RSK3 protein activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA2/RSK3 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Bioactivity
(HTRF assay for RPS6KA2/RSK3 protein activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA2/RSK3 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Bioactivity
(HTRF assay for RPS6KA2/RSK3 protein activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA2/RSK3 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant RPS6KA2/RSK3 protein 10% SDS-PAGE Coomassie staining MW: 84.5 kDa Purity: >= 90%)
SDS-PAGE
(Recombinant RPS6KA2/RSK3 protein 10% SDS-PAGE Coomassie staining MW: 84.5 kDa Purity: >= 90%)
Related Product Information for RPS6KA2/RSK3 active protein
RPS6KA2 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. RPS6KA2 acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. Diseases associated with RPS6KA2 include Coffin-Lowry Syndrome and Dyskeratosis Congenita, Autosomal Dominant 3.
Product Categories/Family for RPS6KA2/RSK3 active protein
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Product Notes
The RPS6KA2/RSK3 (Catalog #AAA60321) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPS6KA2/RSK3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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