Bioactivity
(HTRF assay for RPS6KB1 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
RPS6KB1 active protein
Recombinant RPS6KB1 protein
Purity
>=95%
Synonyms
RPS6KB1; N/A; Recombinant RPS6KB1 protein; Ribosomal Protein S6 Kinase B1; RPS6KB1 active protein
Host
Baculovirus
Purity/Purification
>=95%
Form/Format
Recombinant RPS6KB1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Protein Details
Recombinant RPS6KB1 protein was expressed in baculovirus expression system as the full length protein (accession number NP_003152.1) with a N-terminal DYKDDDDK tag. The molecular weight of the protein is 60.4 kDa.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Bioactivity
(HTRF assay for RPS6KB1 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Bioactivity
(HTRF assay for RPS6KB1 activity 1 uM STK S3 substrate was incubated with different concentrations of RPS6KB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant RPS6KB1 protein 10% SDS-PAGE Coomassie staining MW: 60.4 kDa Purity: >= 95%)
SDS-PAGE
(Recombinant RPS6KB1 protein 10% SDS-PAGE Coomassie staining MW: 60.4 kDa Purity: >= 95%)
Related Product Information for RPS6KB1 active protein
RPS6KB1 (Ribosomal Protein S6 Kinase B1) is a serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression.RPS6KB1 regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B.
Product Categories/Family for RPS6KB1 active protein
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Product Notes
The RPS6KB1 (Catalog #AAA60322) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPS6KB1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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