SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Caspase-3 Active Protein | CASP3 active protein
Recombinant Caspase-3
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Caspase-3; N/A; Recombinant Caspase-3; CPP32; CPP32B; SCA-1; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3 active protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In 20mM HEPES Buffer (pH 7.5) Containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% Glycerol.
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
29-277aa
Sequence
MSGISLDNSY KMDYPEMGLC IIINNKNFHK STGMTSRSGT DVDAANLRET FRNLKYEVRN KNDLTREEIV ELMRDVSKED HSKRSSFVCV LLSHGEEGII FGTNGPVDLK KITNFFRGDR CRSLTGKPKL FIIQACRGTE LDCGIETDSG VDDDMACHKI PVEADFLYAY STAPGYYSWR NSKDGSWFIQ SLCAMLKQYA DKLEFMHILT RVNRKVATEF ESFSFDATFH AKKQIPCIVS MLTKELYFYH HHHHHH
Sequence Length
277
Applicable Applications for CASP3 active protein
Enzyme Activity, SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ser 29
Biological Activity
Specific activity is > 5,000 pmol/min/ug. One unit will liberate 1 pmoles of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for CASP3 active protein
Caspase-3, also known as caspase-3 isoform a, is a member of the cysteine-aspartic acid protease (caspase) family of enzymes. This protein is most abundant in cell lines of lymphocytic origin. Also, it activates caspases 6 and 7 and the protein itself is processed and is activated by caspases 8, 9, and 10. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits that dimerize to form the active enzyme. Members of the caspase family of proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Recombinant Human Caspase-3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CASP3 active protein
References
Walsh JG., et al, (2008) Proc. Natl. Scad. Sci. USA 105:12815. Fernandes-Alnemri T., et al, (1994) J. Biol. Chem. 269:30761-30764.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29.4kDa (256aa)
NCBI Official Full Name
caspase-3 isoform a preproprotein
NCBI Official Synonym Full Names
caspase 3
NCBI Official Symbol
CASP3
NCBI Official Synonym Symbols
CPP32; SCA-1; CPP32B
NCBI Protein Information
caspase-3
UniProt Protein Name
Caspase-3
UniProt Gene Name
CASP3
UniProt Synonym Gene Names
CPP32; CASP-3; CPP-32; SCA-1
UniProt Entry Name
CASP3_HUMAN
Similar Products
Product Notes
The CASP3 casp3 (Catalog #AAA48316) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 29-277aa. AAA Biotech's Caspase-3 can be used in a range of immunoassay formats including, but not limited to, Enzyme Activity, SDS-PAGE. Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSGISLDNSY KMDYPEMGLC IIINNKNFHK STGMTSRSGT DVDAANLRET FRNLKYEVRN KNDLTREEIV ELMRDVSKED HSKRSSFVCV LLSHGEEGII FGTNGPVDLK KITNFFRGDR CRSLTGKPKL FIIQACRGTE LDCGIETDSG VDDDMACHKI PVEADFLYAY STAPGYYSWR NSKDGSWFIQ SLCAMLKQYA DKLEFMHILT RVNRKVATEF ESFSFDATFH AKKQIPCIVS MLTKELYFYH HHHHHH. It is sometimes possible for the material contained within the vial of "Caspase-3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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