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product-image-AAA257845_AD13.png Application Data (Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 ug/mL in the presence of 14 ng/mL rhIGF-II.)

IGFBP5 active protein

IGFBP5 Protein, Human, Recombinant (His Tag)

Gene Names
IGFBP5; IBP5
Purity
>95% as determined by SDS-PAGE
Synonyms
IGFBP5; N/A; IGFBP5 Protein, Human, Recombinant (His Tag); Human IGFBP5/IGFBP-5 Protein (His Tag); IBP5 Protein, Human; insulin-like growth factor binding protein 5; IGFBP5 active protein
Ordering
Host
Baculovirus-Insect Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, pH8.0, 300mM NaCl, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Glu272
Species
Human
Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5ug/mL in the presence of 14ng/mL rhIGF-II.
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Leu 21
Tag
C-His
Protein Construction
A DNA sequence encoding the human IGFBP5 (NP_000590.1)(Met1-Glu272) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

Application Data

(Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 ug/mL in the presence of 14 ng/mL rhIGF-II.)

product-image-AAA257845_AD13.png Application Data (Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 ug/mL in the presence of 14 ng/mL rhIGF-II.)

SDS-PAGE

product-image-AAA257845_SDS_PAGE15.jpg SDS-PAGE

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,570 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 5
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 5
NCBI Official Symbol
IGFBP5
NCBI Official Synonym Symbols
IBP5
NCBI Protein Information
insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5
UniProt Protein Name
Insulin-like growth factor-binding protein 5
UniProt Gene Name
IGFBP5
UniProt Synonym Gene Names
IBP5; IBP-5; IGF-binding protein 5; IGFBP-5
UniProt Entry Name
IBP5_HUMAN

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Product Notes

The IGFBP5 igfbp5 (Catalog #AAA257845) is an Active Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu27 2. It is sometimes possible for the material contained within the vial of "IGFBP5, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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