Monkey B-Cell Activation Factor ELISA Kit | BAFF elisa kit

Monkey B-Cell Activation Factor ELISA Kit

Cat. Num. AAA17036

• Gene Names: bcl2a; bcl2
• Reactivity: Monkey
• Synonyms: B-Cell Activation Factor; N/A; Monkey B-Cell Activation Factor ELISA Kit; BAFF elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of BAFF. No significant cross-reactivity or interference between BAFF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BAFF and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for BAFF elisa kit

Principle of the Assay: BAFF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BAFF antibody and an BAFF-HRP conjugate. The assay sample and buffer are incubated together with BAFF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAFF concentration since BAFF from samples and BAFF-HRP conjugate compete for the anti-BAFF antibody binding site. Since the number of sites is limited, as more sites are occupied by BAFF from the sample, fewer sites are left to bind BAFF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAFF concentration in each sample is interpolated from this standard curve.

Intended Uses: This BAFF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey BAFF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for BAFF elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 71834668
• NCBI GeneID: 570772
• NCBI Accession #: NP_001025424.1
• NCBI GenBank Nucleotide #: NM_001030253.2
• NCBI Official Full Name: Bcl2
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 2a
• NCBI Official Symbol: bcl2a
• NCBI Official Synonym Symbols: bcl2
• NCBI Protein Information: Bcl2; zBlp2; B-cell leukemia/lymphoma 2; B-cell leukemia/lymphoma 2a