SDS-PAGE
(>95% as determined by reducing SDS-PAGE)
Streptomyces Hygroscopicus Bar Recombinant Protein | bar recombinant protein
Recombinant Streptomyces Hygroscopicus Bar Protein
Purity
>95% as determined by reducing SDS-PAGE.
Synonyms
Streptomyces Hygroscopicus Bar; N/A; Recombinant Streptomyces Hygroscopicus Bar Protein; Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar; bar recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 um filtered solution of 12.5mM Tris-HCl, 50mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween 80, 2mM DTT, 1mM EDTA, pH8.5.
Sequence
Met1-Ile183
Sequence Length
183
Species
Streptomyces Hygroscopicus
Reconstitution
We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Preparation and Storage
Lyophilized protein should be stored at < -20°C, Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
This products is provided as lyophilized powder which is shipped with ice packs.
This products is provided as lyophilized powder which is shipped with ice packs.
SDS-PAGE
(>95% as determined by reducing SDS-PAGE)
SDS-PAGE
(>95% as determined by reducing SDS-PAGE)
Related Product Information for bar recombinant protein
Background: Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Molecular Mass: 20.6kDa
Actual Protein Molecular Mass: 18-20kDa
Actual Protein Molecular Mass: 18-20kDa
NCBI Official Full Name
Phosphinothricin N-acetyltransferase
UniProt Protein Name
Phosphinothricin N-acetyltransferase
UniProt Gene Name
bar
UniProt Synonym Gene Names
PPT N-acetyltransferase
UniProt Entry Name
PAT_STRHY
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Product Notes
The Streptomyces Hygroscopicus Bar bar (Catalog #AAA174712) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ile18 3. It is sometimes possible for the material contained within the vial of "Streptomyces Hygroscopicus Bar, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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