SDS-PAGE
Butyrylcholinesterase (BCHE) Recombinant Protein | BCHE recombinant protein
Recombinant Butyrylcholinesterase (BCHE)
Gene Names
BCHE; E1; CHE1; CHE2
Applications
Western Blot, SDS-Page
Purity
> 95%
Synonyms
Butyrylcholinesterase (BCHE); N/A; Recombinant Butyrylcholinesterase (BCHE); BCHE recombinant protein
Host
E.coli
Purity/Purification
> 95%
Form/Format
10mM PBS, pH7.4, containing 5% trehalose and 0.01% sarcosyl.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
ED DIIIATKNGK VRGMNLTVFG GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW SDIWNATKYA NSCCQNIDQS FPGFHGSEMW NPNTDLSEDC LYLNVWIPAP KPKNATVLIW IYGGGFQTGT
Applicable Applications for BCHE recombinant protein
WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Reconsitution
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Residues
Glu29~Thr150
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
8.5
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dateunder appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dateunder appropriate storage condition.
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 14.8kDa
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
cholinesterase
NCBI Official Synonym Full Names
butyrylcholinesterase
NCBI Official Symbol
BCHE
NCBI Official Synonym Symbols
E1; CHE1; CHE2
NCBI Protein Information
cholinesterase; cholinesterase 1; choline esterase II; pseudocholinesterase; butyrylcholine esterase; cholinesterase (serum) 2; acylcholine acylhydrolase
UniProt Protein Name
Cholinesterase
UniProt Gene Name
BCHE
UniProt Synonym Gene Names
CHE1
UniProt Entry Name
CHLE_HUMAN
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Product Notes
The BCHE bche (Catalog #AAA134677) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Butyrylcholinesterase (BCHE) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), SDS-PAGE, Immunogen, Positive Control. Researchers should empirically determine the suitability of the BCHE bche for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ED DIIIATKNGK VRGMNLTVFG GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW SDIWNATKYA NSCCQNIDQS FPGFHGSEMW NPNTDLSEDC LYLNVWIPAP KPKNATVLIW IYGGGFQTGT. It is sometimes possible for the material contained within the vial of "Butyrylcholinesterase (BCHE), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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