Monkey beta2 Microglobulin ELISA Kit | beta2-MG elisa kit

Monkey beta2 Microglobulin ELISA Kit

Cat. Num. AAA17033

• Reactivity: Monkey
• Synonyms: beta2 Microglobulin; N/A; Monkey beta2 Microglobulin ELISA Kit; Monkey b2 Microglobulin ELISA Kit; beta2-MG elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of beta2MG. No significant cross-reactivity or interference between beta2MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between beta2MG and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ?g/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for beta2-MG elisa kit

Principle of the Assay: beta2MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-beta2MG antibody and an beta2MG-HRP conjugate. The assay sample and buffer are incubated together with beta2MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the beta2MG concentration since beta2MG from samples and beta2MG-HRP conjugate compete for the anti-beta2MG antibody binding site. Since the number of sites is limited, as more sites are occupied by beta2MG from the sample, fewer sites are left to bind beta2MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The beta2MG concentration in each sample is interpolated from this standard curve.

Intended Uses: This beta2MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey beta2MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for beta2-MG elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 290491208
• NCBI GeneID: 100379556
• NCBI Accession #: NP_001166327.1
• NCBI GenBank Nucleotide #: NM_001172856.1
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Symbol: B2m
• NCBI Protein Information: beta-2-microglobulin; beta2 microglobulin