Standard Curve (Sample)
Human Biglycan ELISA Kit | BGN elisa kit
Human Biglycan ELISA Kit
Gene Names
BGN; PGI; DSPG1; PG-S1; SLRR1A
Reactivity
Human
Applications
ELISA
Synonyms
Biglycan; N/A; Human Biglycan ELISA Kit; SLRR1A; Bone/cartilage proteoglycan I; PG-S1; BGN elisa kit
Reactivity
Human
Specificity
The Human Biglycan ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Biglycan proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
368
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for BGN elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for BGN elisa kit
Principle of the Assay||The Human Biglycan ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Biglycan in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Biglycan coated on a 96-well plate. Standards and samples are pipetted into the wells and Biglycan present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Biglycan antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Biglycan bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.
Background/Introduction: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,654 Da
NCBI Official Full Name
Biglycan
NCBI Official Synonym Full Names
biglycan
NCBI Official Symbol
BGN
NCBI Official Synonym Symbols
PGI; DSPG1; PG-S1; SLRR1A
NCBI Protein Information
biglycan
UniProt Protein Name
Biglycan
UniProt Gene Name
BGN
UniProt Synonym Gene Names
SLRR1A
UniProt Entry Name
PGS1_HUMAN
Similar Products
Product Notes
The Human BGN bgn (Catalog #AAA261728) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261728 ELISA Kit recognizes Human BGN. AAA Biotech's Biglycan can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the BGN bgn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biglycan, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.