Structure
(PKH 26, CAS 154214-55-8)
PF-04577806 biochemical
PF-04577806
Purity
98%
Synonyms
PF-04577806; N/A; PF-04577806 biochemical
Purity/Purification
98%
CAS
1072100-81-2
Targets&IC50
PKCalpha:2.4 nM (IC50), PKCdelta:586 nM (IC50), PKCtheta:29.5 nM (IC50), PKCgamma:45.9 nM (IC50), PKCbetaI:8.1 nM (IC50), PKCepsilon:522 nM (IC50), PKCbetaII:6.9 nM (IC50)
Solubility
<1mg/ml refers to the product slightly soluble or insoluble
In vitro
PF-04577806 (0.001-10 uM; 10 min) inhibits PKC activity in diabetic rat retinal lysates, with IC 50 of 0.18 uM[1]. PF-04577806 (0.12-10 uM; pretreated for 60 min) inhibits phorbol myristate acetate-stimulated phosphorylation of ERK1/2 in Jurkat cells, with an IC 50 of 0.28 uM[1]. PF-04577806 (0.001-10 uM; pretreated for 1 h) inhibits phosphorylation of SHP2 in HEK293 cells, with an IC 50 of 5.8 nM. PF-04577806 concentration-dependently inhibits interleukin 8 release from phorbol myristate acetate-stimulated HEK293 cells, with an IC 50 of 0.12 uM[1]. PF-04577806 (1 uM; 48 h) shows low cytotoxicity against human umbilical vein endothelial cells with remaining cell viability at 100.5% viable[1]. Western Blot Analysis[1]Cell Line: Jurkat T cells Concentration: 0, 0.12, 0.37, 1.11, 3.33, 10.0 uM Incubation Time: Pretreated for 1 hours. Result: Showed a dose-dependent decrease in phospho-ERK1/2 but not total ERK1/2.
Preparation and Storage
Powder: -20 degree C for 3 years
In solvent: -80 degree C for 2 years
In solvent: -80 degree C for 2 years
Structure
(PKH 26, CAS 154214-55-8)
Structure
(PKH 26, CAS 154214-55-8)
Structure
(PKH 26, CAS 154214-55-8)
Structure
(PKH 26, CAS 154214-55-8)
Related Product Information for PF-04577806 biochemical
PF-04577806 is a potent, selective and ATP competitive PKC inhibitor. PF-04577806 shows potent inhibitory activity towards PKCalpha, PKCbetaI, PKCbetaII, PKCgamma, and PKCtheta with IC 50 s of 2.4 nM, 8.1 nM, 6.9 nM, 45.9 nM, and 29.5 nM, respectively. PF-04577806 can reverse retinal vascular leakage in diabetic rats.
References
1. Li P, et al. PKH26 can transfer to host cells in vitro and vivo. Stem Cells Dev. 2013;22(2):340-344.
2. Fischer K, et al. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity. Methods. 2003;31(2):135-142.
2. Fischer K, et al. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity. Methods. 2003;31(2):135-142.
Similar Products
Product Notes
The PF-04577806 (Catalog #AAA227444) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PF-04577806, Biochemical" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.