Structure
(Kaempferol-3-O-(2''-O-beta-D-glucopyl)-beta-D-rutinoside, CAS 55696-58-7)
SR-0813 biochemical
SR-0813
Purity
98%
Synonyms
SR-0813; N/A; SR-0813 biochemical
Purity/Purification
98%
CAS
2597186-19-9
Targets&IC50
AF9 YEATS domain:311 nM (IC50), AF9 YEATS domain:76 nM (EC50), ENL YEATS domain:205 nM (EC50), ENL YEATS domain:25 nM (IC50), ENL YEATS domain:30 nM (Kd), MAP3K19:3.5 uM (Kd)
Solubility
<1mg/ml refers to the product slightly soluble or insoluble
In vitro
SR-0813 (compound 10; 0, 1, 10 uM; 4 h) dose-dependent evicts ENL from known ENL binding sites, including the HOXA10 gene body and MYB promoter in MV4;11 cells[1]. SR-0813 (0, 1, 10 uM; 0, 24, 48, 72 h) downregulates the transcript levels of HOXA9, MEIS1, and MYC, and increases the abundance of the ITGAM transcript in MV4;11 cells[1]. SR-0813 (0, 1, 10 uM; approximately 2 weeks) inhibits the growth of multiple lineage leukemia (MLL)-fusion leukemia cell lines (MV4;11, MOLM-13, OCI/AML-2) and HB11;19 cells[1]. SR-0813 (1 uM, 4h) does not elicit global changes in gene expression in MV4;11 cells, but produces a strikingly selective suppression of ENL target genes[1]. Cell Proliferation Assay[1]Cell Line: MLL-fusion leukemia cell lines: MV4;11 (MLL-AF4 AML), MOLM-13 (MLL-AF9 AML), and OCI/AML-2 (MLL-AF6 AML), which are sensitive to the genetic loss of ENL. HB11;19 cells, which harboring an MLL-ENL fusion. Concentration: 0, 1, 10 uM Incubation Time: Approximately 2 weeks. Result: Inhibited the growth of cells.
Preparation and Storage
Powder: -20 degree C for 3 years
In solvent: -80 degree C for 2 years
In solvent: -80 degree C for 2 years
Structure
(Kaempferol-3-O-(2''-O-beta-D-glucopyl)-beta-D-rutinoside, CAS 55696-58-7)
Structure
(Kaempferol-3-O-(2''-O-beta-D-glucopyl)-beta-D-rutinoside, CAS 55696-58-7)
Structure
(Kaempferol-3-O-(2''-O-beta-D-glucopyl)-beta-D-rutinoside, CAS 55696-58-7)
Structure
(Kaempferol-3-O-(2''-O-beta-D-glucopyl)-beta-D-rutinoside, CAS 55696-58-7)
Related Product Information for SR-0813 biochemical
SR-0813 is a potent and selective ENL/AF9 YEATS domain inhibitor. SR-0813 has IC 50 and EC 50 values of 25 nM and 205 nM for ENL YEATS domain, respectively. SR-0813 has IC 50 and EC 50 values of 311 nM and 76 nM (CETSA) for AF9 YEATS domain, respectively. SR-0813 binds MAP3K19 with over 100-fold lower affinity (K d =3.5 uM) than ENL YEATS (K d =30 nM). SR-0813 can be used for the research of acute leukemia.
References
1. Jung-HuiChen, et al. Extraction and purification of flavanone glycosides and kaemferol glycosides from defatted Camellia oleifera seeds by salting-out using hydrophilic isopropanol. Separation and Purification Technology Volume 67, Issue 1, 18 May 2009, Pages 31-37.
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Product Notes
The SR-0813 (Catalog #AAA228525) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SR-0813, Biochemical" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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