Bone Morphogenetic protein-7 Recombinant Protein | BMP-7 recombinant protein

Human Bone Morphogenetic protein-7 Recombinant

Cat. Num. AAA76056

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Bone Morphogenetic protein-7; N/A; Human Bone Morphogenetic protein-7 Recombinant; bmp7; bmp-7; op1; BMP-7 recombinant protein

• Host: N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant BMP-7 was lyophilized from a 0.2 um filtered PBS solution pH 7.4.
• Sequence: MHVRSLRAAA PHSFVALWAP LFLLRSALAD FSLDNEVHSS FIHRRLRSQE RREMQREILS ILGLPHRPRP HLQGKHNSAP MFMLDLYNAM AVEEGGGPGG QGFSYPYKAV FSTQGPPLAS LQDSHFLTDA DMVMSFVNLV EHDKEFFHPR YHHREFRFDL SKIPEGEAVT AAEFRIYKDY IRERFDNETF RISVYQVLQE HLGRESDLFL LDSRTLWASE EGWLVFDITA TSNHWVVNPR HNLGLQLSVE TLDGQSINPK LAGLIGRHGP QNKQPFMVAF FKATEVHFRS IRSTGSKQRS QNRSKTPKNQ EALRMANVAE NSSSDQRQAC KKHELYVSFR DLGWQDWIIA PEGYAAYYCE GECAFPLNSY MNATNHAIVQ TLVHFINPET VPKPCCAPTQ LNAISVLYFD DSSNVILKKY RNMVVRACGC H
• Sequence Length: 431

• Source: Optimized DNA sequence encoding Human Bone Morphogenetic protein-7 mature chain was expressed in E. coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human BMP-7 is generated by the proteolytic removal of the signal peptide; and propeptide, the molecule has a calculated molecular mass of approximately 17 kDa.; ; Recombinant BMP-7 is a disulfide-linked homodimeric protein consisting of two 139 amino acid residue subunits, and migrates as an approximately 30 kDa protein under under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for BMP-7 recombinant protein

BMP-7 (osteogenic protein- 1) is produced in the kidney and in the nervous system. It is produced also by human fetal trophoblasts in vivo and may be involved in the regulation of the synthesis of chorionic gonadotropin and progesterone by trophoblasts. BMP7 induces new bone growth, and forms heterodimers with BMP2A. The factor is a member of the TGF family of proteins.

Product Categories/Family for BMP-7 recombinant protein

Cytokines

References

• differential regulation of e-cadherin and -smooth muscle actin by bmp 7 in human renal proximal tubule epithelial cells and its implication in renal fibrosis. .Am J Physiol Renal Physiol, Sep 2009; 10.1152/ajprenal.90539.2008.
• heparin cross-linked collagen coated tcp/ha granules as a carrier for BMP-7 .J Bone Joint Surg Br Proceedings, Sep 2009; 91-B: 445.
• BMP-7 fails to attenuate tgf-β1-induced epithelial-to-mesenchymal transition in human proximal tubule epithelial cells .Nephrol. Dial. Transplant., May 2009; 24: 1406 - 1416.
• the treatment of recalcitrant long bone non-unions with bone morphogenetic protein-7 (BMP-7) .J Bone Joint Surg Br Proceedings, May 2009; 91-B: 268.
• BMP-7 induces wound healing without neovascularization and opacification in a corneal alkali injured mouse model .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4549.