Standard Curve (Sample)
Thyroxine (T4) ELISA Kit | T4 elisa kit
Thyroxine (T4) ELISA Kit
Reactivity
Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Swine
Applications
ELISA
Synonyms
Thyroxine (T4); N/A; Thyroxine (T4) ELISA Kit; T4 elisa kit
Reactivity
Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Swine
Specificity
Thyroxine (T4) ELISA Kit has high sensitivity and excellent specificity for detection of T4. Nosignificant cross-reactivity or interference between T4 and analogues was observed.
Assay Type
Competitive
Samples
Serum, Plasma
Detection Range
20 ng/mL-320 ng/mL
Sensitivity
10 ng/mL
Applicable Applications for T4 elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision: The CV (%)?15%.
Inter-assay Precision
Inter-assay Precision :The CV (%)?15%
Preparation and Storage
The unopened kit should be stored at 2 - 8 degree C. After opening, please store refer to protocols.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for T4 elisa kit
Principle of the Assay: T4 is a useful marker for the identification of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroidpatients and is increased in hyperthyroid patients. The major form of thyroid hormone in the blood is thyroxine (T4), which has alonger half-life than T3. In humans, the ratio of T4 to T3 released into the blood is between 14:1 and 20:1. T4 is converted to theactive T3 (three to four times more potent than T4) within cells by deiodinases (5'-iodinase). These are further processed bydecarboxylation and deiodination to produce iodothyronamine (T1a) and thyronamine (T0a). All three isoforms of the deiodinasesare selenium-containing enzymes, thus dietary selenium is essential for T3 production. Edward Calvin Kendall was responsible forthe isolation of thyroxine in 1915.Thyroxine (T4) ELISA Kit employs the competitive inhibition enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples areadded to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4(Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples,the less antibody bound by Biotin-conjugated T4. After washing, Avidin-HRP is added to the wells. Substrate solution is added tothe wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensityof the color is measured.
Product Categories/Family for T4 elisa kit
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Product Notes
The Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Swine T4 (Catalog #AAA332601) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332601 ELISA Kit recognizes Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Swine T4. AAA Biotech's Thyroxine (T4) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the T4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroxine (T4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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