Bovine Complement Component 3 (C3) ELISA Kit | C3 elisa kit

Cattle Complement Component 3 (C3) ELISA Kit

Cat. Num. AAA184089

• Gene Names: C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• Reactivity: Bovine
• Synonyms: Complement Component 3 (C3); N/A; Cattle Complement Component 3 (C3) ELISA Kit; ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein; C3 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 3 (C3).; No significant cross-reactivity or interference between Complement Component 3 (C3) and analogues was observed.
• Sequence Length: 1663
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.28ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3 (C3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3 (C3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for C3 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complement Component 3 (C3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complement Component 3 (C3) and unlabeled Complement Component 3 (C3) (Standards or samples) with the pre-coated antibody specific to Complement Component 3 (C3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complement Component 3 (C3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complement Component 3 (C3) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 152012784
• NCBI GeneID: 718
• Molecular Weight: 187,148 Da
• NCBI Official Full Name: Complement component 3
• NCBI Official Synonym Full Names: complement C3
• NCBI Official Symbol: C3
• NCBI Official Synonym Symbols: ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• NCBI Protein Information: complement C3
• UniProt Protein Name: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: CPAMD1; C3bc; ASP