Standard Curve (Sample)
Bovine Sp100 Nuclear Antigen ELISA Kit | Sp100 elisa kit
Bovine Sp100 Nuclear Antigen ELISA Kit
Gene Names
SP100; lysp100b
Reactivity
Bovine
Synonyms
Sp100 Nuclear Antigen; N/A; Bovine Sp100 Nuclear Antigen ELISA Kit; Sp100 elisa kit
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This Sp100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Sp100. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Sp100 elisa kit
Principle of the assay: Sp100 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Sp100 antibody and an Sp100-HRP conjugate. The assay sample and buffer are incubated together with Sp100-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Sp100 concentration since Sp100 from samples and Sp100-HRP conjugate compete for the anti-Sp100 antibody binding site. Since the number of sites is limited, as more sites are occupied by Sp100 from the sample, fewer sites are left to bind Sp100-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Sp100 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Sp100 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
50,032 Da
NCBI Official Full Name
Sp100, partial
NCBI Official Synonym Full Names
SP100 nuclear antigen
NCBI Official Symbol
SP100
NCBI Official Synonym Symbols
lysp100b
NCBI Protein Information
nuclear autoantigen Sp-100; speckled 100 kDa; SP100-HMG nuclear autoantigen; nuclear dot-associated Sp100 protein
UniProt Protein Name
Nuclear autoantigen Sp-100
UniProt Gene Name
SP100
UniProt Entry Name
SP100_HUMAN
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Product Notes
The Bovine Sp100 sp100 (Catalog #AAA92048) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA92048 ELISA Kit recognizes Bovine Sp100. It is sometimes possible for the material contained within the vial of "Sp100 Nuclear Antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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