Human Anti bullous pemphigoid 180 antibody ELISA Kit | BP180 Ab elisa kit

Human Anti bullous pemphigoid 180 antibody ELISA Kit

Cat. Num. AAA84934

• Gene Names: COL17A1; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
• Reactivity: Human
• Synonyms: Anti bullous pemphigoid 180 antibody; N/A; Human Anti bullous pemphigoid 180 antibody ELISA Kit; BP180 Ab elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BP180-Ab. No significant cross-reactivity or interference between BP180-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BP180-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for BP180 Ab elisa kit

Principle of the Assay: BP180-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a BP180 antigen and an BP180-Ab-HRP conjugate. The assay sample and buffer are incubated together with BP180-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BP180-Ab concentration since BP180-Ab from samples and BP180-Ab-HRP conjugate compete for the BP180 antigen binding site. Since the number of sites is limited, as more sites are occupied by BP180-Ab from the sample, fewer sites are left to bind BP180-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BP180-Ab concentration in each sample is interpolated from this standard curve.

Intended Uses: This BP180-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BP180-Ab. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for BP180 Ab elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1877435
• NCBI GeneID: 1308
• Molecular Weight: 142,344 Da
• NCBI Official Full Name: 180 kDa bullous pemphigoid antigen 2/type XVII collagen
• NCBI Official Synonym Full Names: collagen, type XVII, alpha 1
• NCBI Official Symbol: COL17A1
• NCBI Official Synonym Symbols: BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
• NCBI Protein Information: collagen alpha-1(XVII) chain; collagen alpha-1(XVII) chain; alpha 1 type XVII collagen; type XVII collagen alpha-1; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180))
• UniProt Protein Name: Collagen alpha-1(XVII) chain
• UniProt Gene Name: COL17A1
• UniProt Synonym Gene Names: BP180; BPAG2; LAD-1; 97 kDa LAD antigen; 97-LAD; LABD97
• UniProt Entry Name: COHA1_HUMAN