Application Data
(HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
BRD2 recombinant protein
Recombinant BRD2 protein
Purity
>=95% by SDS-PAGE.
Synonyms
BRD2; N/A; Recombinant BRD2 protein; Bromodomain Containing Protein 2; RING3; FSHRG1; NAT;FSRG1; RNF3; FSH; D6S113E; BRD2 recombinant protein
Host
Baculovirus
Purity/Purification
>=95% by SDS-PAGE.
Form/Format
Recombinant BRD2 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant BRD2 protein that includes full length of human BRD2 protein (accession number NP_001106653.1) was expressed in Sf9 cells and contains an N-terminal His and DYKDDDDK tag with a molecular weight of 92.5kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant BRD2 protein 10% SDS-PAGE Coomassie staining MW: 92.5kDa Purity: >=95%)
SDS-PAGE
(Recombinant BRD2 protein 10% SDS-PAGE Coomassie staining MW: 92.5kDa Purity: >=95%)
Related Product Information for BRD2 recombinant protein
Bromodomain-containing protein 2 (BRD2), also known as RING3, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.
Product Categories/Family for BRD2 recombinant protein
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Product Notes
The BRD2 (Catalog #AAA60412) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRD2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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