SDS-PAGE
(> 95% as determined by reducing SDS-PAGE)
Biotinidase / Biotinase / BTD Recombinant Protein | BTD recombinant protein
Recombinant Human Biotinidase / Biotinase / BTD Protein (His tag)
Purity
> 95 % as determined by reducing SDS-PAGE
Synonyms
Biotinidase / Biotinase / BTD; N/A; Recombinant Human Biotinidase / Biotinase / BTD Protein (His tag); BTD; BTD recombinant protein
Host
HEK293 Cells
Purity/Purification
> 95 % as determined by reducing SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
Sequence Length
543
Species
Human
Sequence
Met1 - Asp543
Tag
C-His
Bio-Activity
Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmolmin/ug.
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Reconstitution
We recommend centrifuging the vial before opening to rbing the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Concentration is measured by UV-Vis
Preparation and Storage
Generally, lyohphilized proteins are stable for up to 12 months when stored at - 20 degrees to -80 degrees. Reconstituted protein solution can be stored at 4-8 degrees C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degrees C for 3 months.
SDS-PAGE
(> 95% as determined by reducing SDS-PAGE)
SDS-PAGE
(> 95% as determined by reducing SDS-PAGE)
Related Product Information for BTD recombinant protein
Background: Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
Description: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
Description: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 58.2 kDa
Observed MW: 66-76 kDa
Observed MW: 66-76 kDa
NCBI Official Full Name
biotinidase isoform 3
NCBI Official Synonym Full Names
biotinidase
NCBI Official Symbol
BTD
NCBI Protein Information
biotinidase
UniProt Protein Name
Biotinidase
UniProt Gene Name
BTD
UniProt Synonym Gene Names
Biotinase
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Product Notes
The BTD btd (Catalog #AAA173522) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Biotinidase / Biotinase / BTD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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