Canine Thromboglobulin, Beta ELISA Kit | bTG elisa kit
Canine Thromboglobulin, Beta ELISA Kit
Reactivity
Canine
Synonyms
Thromboglobulin, Beta; N/A; Canine Thromboglobulin, Beta ELISA Kit; bTG elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for bTG elisa kit
Intended Uses: This betaTG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine betaTG. This ELISA kit for research use only!
Principle of the Assay: betaTG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaTG antibody and an betaTG-HRP conjugate. The assay sample and buffer are incubated together with betaTG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaTG concentration since betaTG from samples and betaTG-HRP conjugate compete for the anti-betaTG antibody binding site. Since the number of sites is limited, as more sites are occupied by betaTG from the sample, fewer sites are left to bind betaTG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaTG concentration in each sample is interpolated from this standard curve.
Principle of the Assay: betaTG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaTG antibody and an betaTG-HRP conjugate. The assay sample and buffer are incubated together with betaTG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaTG concentration since betaTG from samples and betaTG-HRP conjugate compete for the anti-betaTG antibody binding site. Since the number of sites is limited, as more sites are occupied by betaTG from the sample, fewer sites are left to bind betaTG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaTG concentration in each sample is interpolated from this standard curve.
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