1M Tris-HCl, pH6.8 Buffer
1M Tris-HCl, pH6.8
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Product Notes
The 1M Tris-HCl, pH6.8 (Catalog #AAA13526) is a Buffer and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 1M Tris-HCl, pH6.8 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the 1M Tris-HCl, pH6.8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "1M Tris-HCl, pH6.8, Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is 1M Tris-HCl pH 6.8 buffer used for?
1M Tris-HCl pH 6.8 is a concentrated buffer primarily used in the preparation of "stacking gels" for SDS-PAGE and as a component of protein sample loading buffers. Its specific pH is crucial for concentrating proteins into a tight band before they enter the resolving gel. This ensures high resolution and sharp bands in AAA Biotech-supported electrophoresis experiments.
Why is Tris-HCl pH 6.8 commonly used in SDS-PAGE sample buffer?
Tris-HCl at pH 6.8 is used in sample buffers (like Laemmli buffer) to match the pH of the stacking gel. This consistency maintains the ionic environment necessary for the "stacking" effect, where proteins are compressed between leading and terminating ions. Using a high-quality buffer from AAA Biotech ensures that samples are properly prepared for optimal separation during electrophoresis.
What is the difference between Tris-HCl pH 6.8 and pH 8.0 buffers?
The primary difference is the hydrogen ion concentration, which dictates their specific roles in SDS-PAGE. The pH 6.8 buffer is used for the stacking gel to facilitate protein concentration, while the pH 8.8 (or sometimes 8.0) buffer is used for the resolving gel to allow proteins to separate by size. AAA Biotech provides these distinct buffers to maintain the necessary pH gradients for electrophoresis.
Can 1M Tris-HCl pH 6.8 be used for protein electrophoresis?
Yes, it is an essential component of the protein electrophoresis process. While it is not used as the running buffer itself (which is typically Tris-Glycine), it is vital for the gel chemistry and sample preparation stages. Using the 1M Tris-HCl pH 6.8 solution from AAA Biotech ensures that the electrochemical environment of the gel is correctly established for successful protein separation.
How should 1M Tris-HCl pH 6.8 buffer be stored?
1M Tris-HCl pH 6.8 should be stored at room temperature or at 4°C to maintain its stability. It is important to keep the container tightly sealed to prevent evaporation or pH shifts caused by the absorption of CO2. AAA Biotech recommends checking for any signs of precipitation or microbial growth before use to ensure consistent experimental results.
Is Tris-HCl pH 6.8 suitable for protein denaturation or stacking gels?
It is specifically designed for stacking gels. In terms of denaturation, while Tris-HCl itself is not a denaturant, it provides the required pH environment for denaturing agents like SDS and heat to work effectively. By using AAA Biotech’s pH 6.8 buffer in your sample preparation, you ensure that proteins remain appropriately charged and ready for the stacking process in the gel.
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