Mouse Complement 1q ELISA Kit | C1q elisa kit

Mouse Complement 1q ELISA Kit

Cat. Num. AAA16380

• Gene Names: C1QTNF5; CTRP5
• Reactivity: Mouse
• Synonyms: Complement 1q; N/A; Mouse Complement 1q ELISA Kit; C1q elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of C1Q. No significant cross-reactivity or interference between C1Q and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C1Q and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ?g/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for C1q elisa kit

Principle of the Assay: C1Q ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C1Q antibody and an C1Q-HRP conjugate. The assay sample and buffer are incubated together with C1Q-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1Q concentration since C1Q from samples and C1Q-HRP conjugate compete for the anti-C1Q antibody binding site. Since the number of sites is limited, as more sites are occupied by C1Q from the sample, fewer sites are left to bind C1Q-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1Q concentration in each sample is interpolated from this standard curve.

Intended Uses: This C1Q ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C1Q. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for C1q elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 14149712
• NCBI GeneID: 114902
• NCBI Accession #: NP_056460.1
• NCBI GenBank Nucleotide #: NM_015645.4
• Molecular Weight: 25,298 Da
• NCBI Official Full Name: complement C1q tumor necrosis factor-related protein 5
• NCBI Official Synonym Full Names: C1q and tumor necrosis factor related protein 5
• NCBI Official Symbol: C1QTNF5
• NCBI Official Synonym Symbols: CTRP5
• NCBI Protein Information: complement C1q tumor necrosis factor-related protein 5; myonectin; C1q TNF-alpha-related protein 5
• UniProt Protein Name: Complement C1q tumor necrosis factor-related protein 5
• UniProt Gene Name: C1QTNF5
• UniProt Synonym Gene Names: CTRP5; UNQ303/PRO344
• UniProt Entry Name: C1QT5_HUMAN