Canine Cystatin-B (CSTB/CST6/STFB) ELISA Kit | CSTB elisa kit

Canine Cystatin-B (CSTB/CST6/STFB) ELISA Kit

Cat. Num. AAA246173

• Gene Names: CSTB; PME; ULD; CST6; EPM1; STFB; EPM1A
• Reactivity: Canine
• Synonyms: Cystatin-B (CSTB/CST6/STFB); N/A; Canine Cystatin-B (CSTB/CST6/STFB) ELISA Kit; CSTB elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of CSTB/CST6/STFB. No significant cross-reactivity or interference between CSTB/CST6/STFB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSTB/CST6/STFB and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ug/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CSTB elisa kit

Principle of the Assay: CSTB/CST6/STFB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CSTB/CST6/STFB antibody and an CSTB/CST6/STFB-HRP conjugate. The assay sample and buffer are incubated together with CSTB/CST6/STFB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSTB/CST6/STFB concentration since CSTB/CST6/STFB from samples and CSTB/CST6/STFB-HRP conjugate compete for the anti-CSTB/CST6/STFB antibody binding site. Since the number of sites is limited, as more sites are occupied by CSTB/CST6/STFB from the sample, fewer sites are left to bind CSTB/CST6/STFB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSTB/CST6/STFB concentration in each sample is interpolated from this standard curve.

Intended Uses: This CSTB/CST6/STFB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CSTB/CST6/STFB. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for CSTB elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4503117
• NCBI GeneID: 1476
• NCBI Accession #: NP_000091.1
• NCBI GenBank Nucleotide #: NM_000100.3
• Molecular Weight: 11,140 Da
• NCBI Official Full Name: cystatin-B
• NCBI Official Synonym Full Names: cystatin B (stefin B)
• NCBI Official Symbol: CSTB
• NCBI Official Synonym Symbols: PME; ULD; CST6; EPM1; STFB; EPM1A
• NCBI Protein Information: cystatin-B; CPI-B; liver thiol proteinase inhibitor
• UniProt Protein Name: Cystatin-B
• UniProt Gene Name: CSTB
• UniProt Synonym Gene Names: CST6; STFB
• UniProt Entry Name: CYTB_HUMAN