Canine L-selectin ELISA Kit | L-selectin elisa kit

Canine L-selectin ELISA Kit

Cat. Num. AAA93579

• Gene Names: SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• Reactivity: Canine
• Synonyms: L-selectin; N/A; Canine L-selectin ELISA Kit; L-selectin elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of SELL. No significant cross-reactivity or interference between SELL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SELL and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for L-selectin elisa kit

Principle of the Assay: SELL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SELL antibody and an SELL-HRP conjugate. The assay sample and buffer are incubated together with SELL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SELL concentration since SELL from samples and SELL-HRP conjugate compete for the anti-SELL antibody binding site. Since the number of sites is limited, as more sites are occupied by SELL from the sample, fewer sites are left to bind SELL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SELL concentration in each sample is interpolated from this standard curve.

Intended Uses: This SELL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of CanineSELL. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for L-selectin elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 5852072
• NCBI GeneID: 6402
• UniProt Accession #: Q9UJ43; P15023; B2R6Q8; P14151
• Molecular Weight: 43,618 Da
• NCBI Official Full Name: L-selectin
• NCBI Official Synonym Full Names: selectin L
• NCBI Official Symbol: SELL
• NCBI Official Synonym Symbols: TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• NCBI Protein Information: L-selectin; gp90-MEL; pln homing receptor; lymph node homing receptor; lymphocyte adhesion molecule 1; leukocyte surface antigen Leu-8; CD62 antigen-like family member L; leukocyte-endothelial cell adhesion molecule 1
• UniProt Protein Name: L-selectin
• UniProt Gene Name: SELL
• UniProt Synonym Gene Names: LNHR; LYAM1; LAM-1; LECAM1
• UniProt Entry Name: LYAM1_HUMAN