Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit | NT-ProBNP elisa kit
Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
Reactivity
Canine
Synonyms
N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP); N/A; Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit; NT-Pro-BNP; N-BNP; NT-ProBNP elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of noradrenaline. No significant cross-reactivity or interference between noradrenaline and analogues was observed.
Samples
Serum, Plasma, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Competitive
Detection Range
61.7-5,000pg/mL
Sensitivity
25.5pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level noradrenaline were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level noradrenaline were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Standard Curve (Sample)
Related Product Information for NT-ProBNP elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of noradrenaline in serum, plasma, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to noradrenaline has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled noradrenaline and unlabeled noradrenaline (Standards or samples) with the pre-coated antibody specific to noradrenaline. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of noradrenaline in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of noradrenaline in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to noradrenaline has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled noradrenaline and unlabeled noradrenaline (Standards or samples) with the pre-coated antibody specific to noradrenaline. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of noradrenaline in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of noradrenaline in the sample.
