Canine Neurofilament light polypeptide (NEFL) ELISA Kit | NEFL elisa kit

Canine Neurofilament light polypeptide (NEFL) ELISA Kit

Cat. Num. AAA27228

• Gene Names: NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• Reactivity: Canine
• Synonyms: Neurofilament light polypeptide (NEFL); N/A; Canine Neurofilament light polypeptide (NEFL) ELISA Kit; NEFL elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of NEFL. No significant cross-reactivity or interference between NEFL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NEFL and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for NEFL elisa kit

Principle of the Assay: NEFL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NEFL antibody and an NEFL-HRP conjugate. The assay sample and buffer are incubated together with NEFL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFL concentration since NEFL from samples and NEFL-HRP conjugate compete for the anti-NEFL antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFL from the sample, fewer sites are left to bind NEFL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFL concentration in each sample is interpolated from this standard curve.

Intended Uses: This NEFL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NEFL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for NEFL elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 105990539
• NCBI GeneID: 4747
• NCBI Accession #: NP_006149.2
• NCBI GenBank Nucleotide #: NM_006158.4
• Molecular Weight: 61,517 Da
• NCBI Official Full Name: neurofilament light polypeptide
• NCBI Official Synonym Full Names: neurofilament, light polypeptide
• NCBI Official Symbol: NEFL
• NCBI Official Synonym Symbols: NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• NCBI Protein Information: neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subunit 110
• UniProt Protein Name: Neurofilament light polypeptide
• UniProt Gene Name: NEFL
• UniProt Synonym Gene Names: NF68; NFL; NF-L
• UniProt Entry Name: NFL_HUMAN