CD49f Antibody | anti-CD49f antibody

Anti-CD49f Purified

Cat. Num. AAA129241

• Gene Names: ITGA6; CD49f; VLA-6; ITGA6B
• Reactivity: Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD49f; N/A; Anti-CD49f Purified; integrin subunit alpha 6; ITGA6; VLA6alpha; platelet gpIc; anti-CD49f antibody

• Reactivity: Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse
• Isotype: Rat IgG2a
• Clone Number: GoH3
• Specificity: The rat monoclonal antibody GoH3 recognizes an extracellular epitope of CD49f, an alpha6 integrin subunit.
• Form/Format: Purified; Purified by protein-G affinity chromatography.; Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD49f antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
• Immunogen: mouse mammary tumor cells
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human blood thrombocytes stained using anti-CD49f (GoH3) purified antibody (concentration in sample 1,7 ?g/ml, GAM APC, red-filled) from blood thromobocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49f (GoH3) purified antibody (concentration in sample 1,7 ?g/ml, GAM APC).)

Related Product Information for anti-CD49f antibody

CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.

References

*Martignani E, Ala U, Sheehy PA, Thomson PC, Baratta M: Whole transcriptome analysis of bovine mammary progenitor cells by P-Cadherin enrichment as a marker in the mammary cell hierarchy. Comparative Study Sci Rep. 2020 Aug 25;10(1):14183., URL: https://pubmed.ncbi.nlm.nih.gov/32843665/,*Knapp DJ, Hammond CA, Aghaeepour N, Miller PH, Pellacani D, Beer PA, Sachs K, Qiao W, Wang W, Humphries RK, Sauvageau G, Zandstra PW, Bendall SC, Nolan GP, Hansen C, Eaves CJ: Distinct signaling programs control human hematopoietic stem cell survival and proliferation. Blood 2017 Jan 19;129(3):307-318, URL: https://pubmed.ncbi.nlm.nih.gov/27827829/,*Karthaus WR, Iaquinta PJ, Drost J, Gracanin A, van Boxtel R, Wongvipat J, Dowling CM, Gao D, Begthel H, Sachs N, Vries RGJ, Cuppen E, Chen Y, Sawyers CL, Clevers HC: Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell 2014 Sep 25;159(1):163-175., URL: https://pubmed.ncbi.nlm.nih.gov/25201529/,*Ikeda A, Yamamoto T, Mineshiba J, Takashiba S: Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep. 2020 Nov 17;10(1):19959., URL: https://pubmed.ncbi.nlm.nih.gov/33203957/,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,

NCBI and Uniprot Product Information

• NCBI GI #: 119395742
• NCBI GeneID: 3655
• NCBI Accession #: NP_000201.2
• NCBI GenBank Nucleotide #: NM_000210.2
• UniProt Accession #: P23229; Q08443; Q0MRC7; Q14646; Q16508; Q53RX7; Q59HB7; Q86VL6; B2RMU9; B4DG69; B4DKB8; C4AM96
• Molecular Weight: 126,633 Da
• NCBI Official Full Name: integrin alpha-6 isoform b
• NCBI Official Synonym Full Names: integrin, alpha 6
• NCBI Official Symbol: ITGA6
• NCBI Official Synonym Symbols: CD49f; VLA-6; ITGA6B
• NCBI Protein Information: integrin alpha-6; integrin alpha6B; CD49 antigen-like family member F
• UniProt Protein Name: Integrin alpha-6
• UniProt Gene Name: ITGA6
• UniProt Entry Name: ITA6_HUMAN